Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Transient and steady-state effects of shear stress on endothelial cell adherens junctions
Autore:
Noria, S; Cowan, DB; Gotlieb, AI; Langille, BL;
Indirizzi:
Toronto Hosp, Res Inst, Vasc Res Lab, Toronto, ON M5T 2S8, Canada Toronto Hosp Toronto ON Canada M5T 2S8 s Lab, Toronto, ON M5T 2S8, Canada Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON, Canada Univ Toronto Toronto ON Canada Lab Med & Pathobiol, Toronto, ON, Canada
Titolo Testata:
CIRCULATION RESEARCH
fascicolo: 6, volume: 85, anno: 1999,
pagine: 504 - 514
SICI:
0009-7330(19990917)85:6<504:TASEOS>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
VE-CADHERIN; BETA-CATENIN; F-ACTIN; TYROSINE PHOSPHORYLATION; INTERCELLULAR-JUNCTIONS; ALPHA-CATENIN; RABBIT AORTA; N-CADHERIN; PLAKOGLOBIN; ADHESION;
Keywords:
shear stress; endothelium; vascular endothelial cadherin; alpha-catenin; beta-catenin; plakoglobin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Langille, BL Toronto Gen Hosp, Vasc Res Lab, 200 Elizabeth St,CCRW 1-856, Toronto, ON M5G 2C4, Canada Toronto Gen Hosp 200 Elizabeth St,CCRW 1-856 Toronto ON Canada M5G 2C4
Citazione:
S. Noria et al., "Transient and steady-state effects of shear stress on endothelial cell adherens junctions", CIRCUL RES, 85(6), 1999, pp. 504-514

Abstract

Endothelial cells exhibit profound changes in cell shape in response to altered shear stress that may require disassembly/reassembly of adherens junction protein complexes that mediate cell-cell adhesion. To test this hypothesis, we exposed confluent porcine aortic endothelial cells to 15 dyne/cm(2) of shear stress for 0, 8.5, 24, or 48 hours, using a parallel plate flow chamber. Cells were fixed and stained with antibodies to vascular endothelial (VE) cadherin, alpha-catenin, beta-catenin, or plakoglobin. Under staticconditions, staining for all proteins was intense and peripheral, forming a nearly continuous band around the cells at cell-cell junctions. After 8.5hours of shear stress, staining was punctate and occurred only at sites ofcontinuous cell attachment. After 24 or 48 hours of shear, staining for VE-cadherin, alpha-catenin, and beta-catenin was intense and peripheral, forming a band of "dashes" (adherens plaques) that colocalized with the ends ofstress fibers that inserted along the lateral membranes of cells. Stainingfor plakoglobin was not observed after 24 hours of shear stress, but returned after 48 hours. Western blot analysis indicated that protein levels of VE-cadherin, alpha-catenin, and plakoglobin decreased, whereas beta-cateninlevels increased after 8.5 hours of shear stress. As cell shape change reached completion (24 to 48 hours), all protein levels were upregulated except for plakoglobin, which remained below control levels. The partial disassembly of adherens junctions we have observed during shear induced changes inendothelial cell shape may have important implications for control of the endothelial permeability barrier and other aspects of endothelial cell function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 05:23:08