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Titolo:
Proline-rich antimicrobial peptide, PR-39 gene transduction altered invasive activity and actin structure in human hepatocellular carcinoma cells
Autore:
Ohtake, T; Fujimoto, Y; Ikuta, K; Saito, H; Ohhira, M; Ono, M; Kohgo, Y;
Indirizzi:
Asahikawa Med Coll, Dept Internal Med 3, Asahikawa, Hokkaido 0788307, Japan Asahikawa Med Coll Asahikawa Hokkaido Japan 0788307 kkaido 0788307, Japan
Titolo Testata:
BRITISH JOURNAL OF CANCER
fascicolo: 3, volume: 81, anno: 1999,
pagine: 393 - 403
SICI:
0007-0920(199910)81:3<393:PAPPGT>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAMMARY EPITHELIAL-CELLS; HEPARAN-SULFATE PROTEOGLYCANS; SURFACE PROTEOGLYCAN; ANTIBACTERIAL PEPTIDES; PIG INTESTINE; BONE-MARROW; SH3 DOMAINS; SYNDECAN; EXPRESSION; BINDING;
Keywords:
antimicrobial peptide; PR-39; actin; hepatocellular carcinoma; syndecan;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Fujimoto, Y Asahikawa Med Coll, Dept Internal Med 3, Nishikagura 4-5, Asahikawa, Hokkaido 0788307, Japan Asahikawa Med Coll Nishikagura 4-5 AsahikawaHokkaido Japan 0788307
Citazione:
T. Ohtake et al., "Proline-rich antimicrobial peptide, PR-39 gene transduction altered invasive activity and actin structure in human hepatocellular carcinoma cells", BR J CANC, 81(3), 1999, pp. 393-403

Abstract

PR-39 is an endogenous proline-rich antimicrobial peptide which induces the synthesis of syndecan-1, a transmembrane heparan sulphate proteoglycan involved in cell-to-matrix interactions and wound healing. Previously, we revealed that the expression of syndecan-1 was reduced in human hepatocellularcarcinomas with high metastatic potential and speculated that syndecan-1 played an important role in inhibition of invasion and metastasis. It is assumed that a modification of this; process with PR-39 and syndecan-1 may result in a new strategy by which it can inhibit the invasion and metastasis. Therefore, we transduced a gene of PR-39 into human hepatocellular carcinoma cell line HLF, which shows a low expression of syndecan-1 and a high in vitro invasive activity, and examined whether this procedure could reduce the invasive activity of tumour cells. In two transfectants with PR-39 gene, the syndecan-1 expression was induced and the invasive activity in type I collagen-coated chamber was inhibited. Moreover, these transfectants showed the suppression of motile activity assayed by phagokinetic tracks in addition to the disorganization of actin filaments observed by a confocal imagingsystem. In contrast, five transfectants with syndecan-1 gene in the HLF cells revealed suppression of invasive activity but did not alter the motile activity and actin structures of the cell. These results suggest that PR-39has functions involved in the suppression of motile activity and alteration of actin structure on human hepatocellular carcinoma cells in addition tothe suppression of invasive activity which might result from the inductionof syndecan-1 expression. (C) 1999 Cancer Research Campaign.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 07:30:15