Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Recombinant human insulin-like growth factor I stimulates all stages of 11-ketotestosterone-induced spermatogenesis in the Japanese eel, Anguilla japonica, in vitro
Autore:
Nader, MR; Miura, T; Ando, N; Miura, C; Yamauchi, K;
Indirizzi:
Hokkaido Univ, Fac Fisheries, Dept Biol, Hakodate, Hokkaido 0418611, JapanHokkaido Univ Hakodate Hokkaido Japan 0418611 te, Hokkaido 0418611, Japan Japan Sci & Technol Corp, PRESTO, Kawaguchi, Saitama 3320012, Japan Japan Sci & Technol Corp Kawaguchi Saitama Japan 3320012 a 3320012, Japan
Titolo Testata:
BIOLOGY OF REPRODUCTION
fascicolo: 4, volume: 61, anno: 1999,
pagine: 944 - 947
SICI:
0006-3363(199910)61:4<944:RHIGFI>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN CHORIONIC-GONADOTROPIN; SERTOLI CELLS; DNA-SYNTHESIS; IGF-I; TROUT; TESTIS; PROLIFERATION; SPERMATOGONIA; EXPRESSION; INDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Nader, MR Hokkaido Univ, Fac Fisheries, Dept Biol, Hakodate, Hokkaido 0418611, Japan Hokkaido Univ Hakodate Hokkaido Japan 0418611 do 0418611, Japan
Citazione:
M.R. Nader et al., "Recombinant human insulin-like growth factor I stimulates all stages of 11-ketotestosterone-induced spermatogenesis in the Japanese eel, Anguilla japonica, in vitro", BIOL REPROD, 61(4), 1999, pp. 944-947

Abstract

In this study, we examined the in vitro effects of insulin-like growth factor I (IGF-I) in the presence or absence of 11-keto-testosterone (11-KT: the spermatogenesis-inducing hormone) on the proliferation of Japanese eel (Anguilla japonica) testicular germ cells. Initially, a short-term culture (15 days) of testicular tissue with only type A and early type B spermatogonia (preproliferated spermatogonia) was carried out in Leibovitz-15 growth medium supplemented with different concentrations of recombinant human IGF (rhIGF)-I or -II in the presence or absence of 10 ng/ml of 11-KT. Late type Bspermatogonia (proliferated spermatogonia) were observed in treatments of 100 ng/ml of both rhIGF-I and -II in combination with 11-KT, indicating theonset and progression of spermatogenesis. In all tested rhIGF-I concentrations (except 0.1 ng/ml) supplemented with 11-KT, late type 8 spermatogonia were detected in at least one individual. Then, we proceeded with an in vitro 45-day culture of testicular tissue with 100 ng/ml of rhIGF-I in the presence or absence of 10 ng/ml of 11-KT to test the long-term effects of rhIGF-I on the spermatogenetic cycle. The presence of all types of germ cells, including spermatozoa, in the testis cultured with the admixture of the twohormones indicated that the germ cells underwent complete spermatogenesis whereas no germ cell proliferation was observed when the rhIGF-I was applied alone. These results suggest that IGF-I in the presence of 11-KT plays anessential role in the onset, progress, and regulation of spermatogenesis in the testis of the Japanese eel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/21 alle ore 03:21:23