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Titolo:
Multidrug resistance and retroviral transduction potential in human small cell lung cancer cell lines
Autore:
Theilade, MD; Gram, GJ; Jensen, PB; Cianfriglia, M; Rorth, M; Hansen, JES;
Indirizzi:
HS Hvidovre Hosp, Dept 144, Infect Dis Lab, DK-2650 Hvidovre, Denmark HS Hvidovre Hosp Hvidovre Denmark DK-2650 Lab, DK-2650 Hvidovre, Denmark Ist Super Sanita, Immunol Lab, I-00161 Rome, Italy Ist Super Sanita RomeItaly I-00161 ta, Immunol Lab, I-00161 Rome, Italy Rigshosp, Dept Oncol, DK-2100 Copenhagen, Denmark Rigshosp Copenhagen Denmark DK-2100 t Oncol, DK-2100 Copenhagen, Denmark
Titolo Testata:
APMIS
fascicolo: 9, volume: 107, anno: 1999,
pagine: 851 - 858
SICI:
0903-4641(199909)107:9<851:MRARTP>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
CULTURE CONDITIONS; TOPOISOMERASE II; LEUKEMIA-VIRUS; TRANSPORTER; CARCINOMA; MRP; PROTEIN; GENE; DNA;
Keywords:
small cell lung cancer; multidrug resistance; Pgp; MRP; LacZ; retroviral vectors; gene therapy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Hansen, JES HS Hvidovre Hosp, Dept 144, Infect Dis Lab, Kettegaard Alle 30, DK-2650 Hvidovre, Denmark HS Hvidovre Hosp Kettegaard Alle 30 Hvidovre Denmark DK-2650
Citazione:
M.D. Theilade et al., "Multidrug resistance and retroviral transduction potential in human small cell lung cancer cell lines", APMIS, 107(9), 1999, pp. 851-858

Abstract

Multidrug resistance (MDR) remains a major problem in the successful treatment of small cell lung cancer (SCLC). New treatment strategies are needed,such as gene therapy specifically targeting the MDR cells in the tumor. Retroviral LacZ gene-containing vectors that were either pseudotyped for the gibbon ape leukemia virus (GALV-1) receptor or had specificity for the amphotropic murine leukemia virus (MLV-A) receptor were used for transduction of five SCLC cell lines differing by a range of MDR mechanisms. Transductionefficiencies in these cell lines were compared by calculating the percentage of blue colonies after X-Gal staining of the cells grown in soft agar. All examined SCLC cell lines were transducible with either vector. Transduction efficiencies varied from 5.7% to 33.5% independent of the presence of MDR. These results indicate that MDR does not severely impair transduction of SCLC cells, and that MLV-A as well as GALV-1 retroviral Vectors are suitable for further development of gene therapy in SCLC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 09:27:48