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Titolo:
Localization of labile posttranslational modifications by electron capturedissociation: The case of gamma-carboxyglutamic acid
Autore:
Kelleher, RL; Zubarev, RA; Bush, K; Furie, B; Furie, BC; McLafferty, FW; Walsh, CT;
Indirizzi:
Cornell Univ, Dept Chem & Biol Chem, Ithaca, NY 14850 USA Cornell Univ Ithaca NY USA 14850 t Chem & Biol Chem, Ithaca, NY 14850 USA Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 em & Mol Pharmacol, Boston, MA 02115 USA Beth Deaconess Israel Hosp, Boston, MA 02115 USA Beth Deaconess Israel Hosp Boston MA USA 02115 Hosp, Boston, MA 02115 USA
Titolo Testata:
ANALYTICAL CHEMISTRY
fascicolo: 19, volume: 71, anno: 1999,
pagine: 4250 - 4253
SICI:
0003-2700(19991001)71:19<4250:LOLPMB>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
ION-CYCLOTRON-RESONANCE; INFRARED MULTIPHOTON DISSOCIATION; IONIZATION MASS-SPECTROMETRY; K-DEPENDENT CARBOXYLATION; COLLISIONAL ACTIVATION; LARGE BIOMOLECULES; FACTOR-IX; PEPTIDES; EXCITATION; PROPEPTIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: McLafferty, FW Cornell Univ, Dept Chem & Biol Chem, Ithaca, NY 14850 USA Cornell Univ Ithaca NY USA 14850 hem, Ithaca, NY 14850 USA
Citazione:
R.L. Kelleher et al., "Localization of labile posttranslational modifications by electron capturedissociation: The case of gamma-carboxyglutamic acid", ANALYT CHEM, 71(19), 1999, pp. 4250-4253

Abstract

Tandem mass spectrometry (MS/MS) of 28 residue peptides harboring gamma-carboxylated glutamic acid residues, a posttranslational modification of several proenzymes of the blood coagulation cascade, using either collisions orinfrared photons results in complete ejection of the gamma-CO2 moieties (-44 Da) before cleavage of peptide-backbone bonds. However, MS/MS using electron capture dissociation (ECD) in a Fourier transform mass spectrometer cleaves backbone bonds without ejecting CO2, allowing direct localization of this labile modification. Sulfated side chains are also retained in ECD backbone fragmentations of a 21-mer peptide, although CAD causes extensive SO3loss. ECD thus is a unique complement to conventional methods for MS/MS, causing less undesirable loss of side-chain functionalities as well as more desirable backbone cleavages.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 05:07:18