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Titolo:
Increased metabolism of infused 1-methylxanthine by working muscle
Autore:
Youd, JM; Newman, JMB; Clark, MG; Appleby, GJ; Rattigan, S; Tong, ACY; Vincent, MA;
Indirizzi:
Univ Tasmania, Dept Biochem, Hobart, Tas 7001, Australia Univ Tasmania Hobart Tas Australia 7001 chem, Hobart, Tas 7001, Australia
Titolo Testata:
ACTA PHYSIOLOGICA SCANDINAVICA
fascicolo: 4, volume: 166, anno: 1999,
pagine: 301 - 308
SICI:
0001-6772(199908)166:4<301:IMOI1B>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFUSED RAT HINDLIMB; SKELETAL-MUSCLE; BLOOD-FLOW; HUMAN LIMBS; EXERCISE; SEROTONIN; OXYGEN; VASOCONSTRICTION; REDISTRIBUTION; INHIBITION;
Keywords:
blood flow; muscle contraction; vasodilation; xanthine oxidase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Clark, MG Univ Tasmania, Dept Biochem, GPO Box 252-58, Hobart, Tas 7001, Australia Univ Tasmania GPO Box 252-58 Hobart Tas Australia 7001 Australia
Citazione:
J.M. Youd et al., "Increased metabolism of infused 1-methylxanthine by working muscle", ACT PHYSL S, 166(4), 1999, pp. 301-308

Abstract

Exogenous substrates for capillary endothelial enzymes have potential as markers for changes in capillary recruitment (albeit nutritive flow). The metabolism of infused 1-methylxanthine (1-MX) to 1-methylurate (1-MU) by capillary endothelial xanthine oxidase of the constant-flow perfused rat hindlimb was shown previously to decrease with oxygen uptake (Vo(2)) when nutritive flow was decreased. In the present study, the metabolism of 1-MX was investigated under conditions when Vo(2) and nutritive flow are known to increase during muscle contraction. The constant-flow red blood cell-perfused rat hindlimb at 37 degrees C was used with sciatic nerve stimulation, and perfusate samples from whole hindlimb and working muscles taken for analysis of oxygen, lactate, 1-MX and 1-MU. Flow to muscle was assessed separately using fluorescent microspheres and was found to increase 2.3-fold to the working muscles while flow to the non-working leg muscles decreased to compensate. The activity of xanthine oxidase of whole muscle extracts was not altered by contraction. Samples from the Vein draining the working muscles, and microsphere measurements of flow, indicated increased lie, (5.5-fold to 249.2 +/- 43.1 mu mol h(-1) g(-1), P < 0.001), and 1-MX conversion (2.5-fold to 1.87 +/- 0.25 mu mol h(-1) g(-1). P < 0.01) (SEM are shown). It is concluded that as 1-MX metabolism parallels Vo(2), this substrate may be a usefulindicator of changes in capillary (nutritive) surface area in muscle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/10/20 alle ore 00:41:39