Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effects of transforming growth factor-beta 1 on renal extracellular matrixcomponents and their regulating proteins
Autore:
Douthwaite, JA; Johnson, TS; Haylor, JL; Watson, P; El Nahas, AM;
Indirizzi:
No Gen Hosp Trust, Sheffield Kidney Inst, Sheffield S5 7AU, S Yorkshire, England No Gen Hosp Trust Sheffield S Yorkshire England S5 7AU Yorkshire, England
Titolo Testata:
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
fascicolo: 10, volume: 10, anno: 1999,
pagine: 2109 - 2119
SICI:
1046-6673(199910)10:10<2109:EOTGF1>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLOMERULAR MESANGIAL CELLS; CATALYZED CROSS-LINKING; FACTOR-BETA; TISSUE INHIBITOR; IV COLLAGENASE; METALLOPROTEINASE INHIBITOR; INTERSTITIAL COLLAGENASE; DIFFERENTIAL REGULATION; OSTEOBLASTIC CELLS; GELATINASE-B;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Johnson, TS No Gen Hosp Trust, Sheffield Kidney Inst, Herries Rd, Sheffield S5 7AU, S Yorkshire, England No Gen Hosp Trust Herries Rd Sheffield S Yorkshire England S5 7AU
Citazione:
J.A. Douthwaite et al., "Effects of transforming growth factor-beta 1 on renal extracellular matrixcomponents and their regulating proteins", J AM S NEPH, 10(10), 1999, pp. 2109-2119

Abstract

Transforming growth factor-beta 1 (TGF-beta 1) is widely regarded as a potent fibrogenic renal growth factor. In cell culture, TGF-beta 1 has been shown to increase various extracellular matrix (ECM) proteins and tissue inhibitors of metalloproteinases (TIMP), while decreasing matrix metalloproteinases (MMP), providing the optimum environment for progressive ECM accumulation. This study, which uses the isolated perfused rat kidney (IPRK), describes for the first time in a whole kidney preparation the action of TGF-beta1 on factors associated with ECM processing. This model allows the study of the intact rat kidney with physiologic cell-cell interactions in the absence of confounding systemic influences. Left kidneys were removed from maleWistar rats by a nonischemic technique and perfused with a sterile, apyrogenic, endotoxin-free perfusate, based on the plasma volume expander Hemaccel (polygeline), at constant pressure in a recirculating IPRK system. Kidneys were perfused for 1 h either with (n = 3) or without (n = 3) recombinant human TGF-beta 1 (20 ng/ml). The effects of perfusion were controlled by comparison with the nonperfused contralateral kidney (n = 6). TGF-beta 1 was measured in the perfusate and urine, at the start and end of the experimentusing an enzyme-linked immunosorbent assay to its biologically active form. After perfusion, sections of the kidneys were analyzed for changes in mRNA by Northern blotting. Significant increases in mRNA for fibronectin (7.5-fold, P < 0.01), heparan sulfate proteoglycan core protein (53-fold, P < 0.001), laminin beta 1 (12-fold, P < 0.001), collagen alpha 1(IV) (17-fold, P< 0.001), collagen alpha 1(III) (fourfold, P < 0.001), and MMP9 (twofold, P < 0.05) were observed after perfusion with TGF-beta 1. Measurement of TIMP1, TIMP2, TIMP3, MMP1, and MMP2 mRNA demonstrated no detectable change, whereas determination of mRNA for tissue transglutaminase, an enzyme capable of cross-linking many ECM components, showed an eightfold increase (P < 0.01). This study suggests that in the IPRK and in the absence of other exogenous growth factors, TGF-beta 1 selectively increases the synthesis of ECM and tissue transglutaminase without changes that would result in the reduction of ECM degradation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 04:12:08