Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effect of probucol on intracellular pH and proliferation of human vascularendothelial cells
Autore:
Komatsu, S; Sawada, S; Tamagaki, T; Tsuda, Y; Kono, Y; Higaki, T; Imamura, H; Tada, Y; Yamasaki, S; Toratani, A; Sato, T; Akamatsu, N; Tsuji, H; Nakagawa, M;
Indirizzi:
Kyoto Prefectural Univ Med, Dept Med 2, Kamigyo Ku, Kyoto 6028566, Japan Kyoto Prefectural Univ Med Kyoto Japan 6028566 Ku, Kyoto 6028566, Japan
Titolo Testata:
JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS
fascicolo: 1, volume: 41, anno: 1999,
pagine: 33 - 41
SICI:
1056-8719(199902)41:1<33:EOPOIP>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; NA+/H+ EXCHANGE; CHEMOTACTIC FACTORS; HUMAN-NEUTROPHILS; ATHEROSCLEROSIS; FIBROBLASTS; ACTIVATION; CALCIUM; RABBIT; PHOSPHOLIPASE-A2;
Keywords:
intracellular pH; cell proliferation; prostacyclin; probucol; LDL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Tamagaki, T Kyoto Prefectural Univ Med, Dept Med 2, Kamigyo Ku, 465 Kawaramachi Hirokoji, Kyoto 6028566, Japan Kyoto Prefectural Univ Med 465 Kawaramachi Hirokoji Kyoto Japan 6028566
Citazione:
S. Komatsu et al., "Effect of probucol on intracellular pH and proliferation of human vascularendothelial cells", J PHARM TOX, 41(1), 1999, pp. 33-41

Abstract

We investigated the effect of probucol on the intracellular pH ([pH]i) andproliferation of human umbilical vein endothelial cells (HUVEC). as well as their production of prostacyclin (PGI(2)). The addition of probucol produced a biphasic shift in [pH]i, with a brief initial acidification followed by a rapid alkaline shift. After pretreatment with EGTA. the initial decrease in [pH]i was abolished, and the subsequent increase was inhibited. Afterpretreatment with amiloride. only the increase of [pH]i was abolished. These results suggest that the probucol-induced increase of [pH]i was mainly dependent on Na+/H+ exchange and partly on extracellular Ca2+. In contrast, the addition of LDL produced a decrease of [pH]i. Under Ca2+-free condition. [pH]i was further decreased by LDL. In cells pretreated with amiloride, however, [pH]i was not further decreased by LDL. It was found that probucol promoted cell proliferation, and LDL inhibited cell proliferation. Additionof probucol also enhanced prostacyclin generation by HUVEC. This enhancement of PGI(2) generation resulted from increased release of Ca2+ from the storage sites. due not only to increased production of inositol 1,4,5-triphosphate (IP3) but also to the increase of [pH]i. These findings may help to explain the antiatheroselerotic action of probucol. (C) 1999 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 01:31:33