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Titolo:
ENDOPLASMIC-RETICULUM RETENTION MEDIATED BY THE TRANSMEMBRANE DOMAIN OF TYPE-II MEMBRANE-PROTEINS SEC12P AND GLUCOSIDASE-1
Autore:
TANG BL; LOW SH; HONG WJ;
Indirizzi:
NATL UNIV SINGAPORE,INST MOL & CELL BIOL,MEMBRANE BIOL LAB,10 KENT RIDGE CRESCENT SINGAPORE 119260 SINGAPORE NATL UNIV SINGAPORE,INST MOL & CELL BIOL,MEMBRANE BIOL LAB SINGAPORE 119260 SINGAPORE UNIV CALIF SAN FRANCISCO,DEPT ANAT SAN FRANCISCO CA 94143
Titolo Testata:
European journal of cell biology
fascicolo: 2, volume: 73, anno: 1997,
pagine: 98 - 104
SICI:
0171-9335(1997)73:2<98:ERMBTT>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANS-GOLGI NETWORK; DIPEPTIDYL PEPTIDASE-IV; DARBY CANINE KIDNEY; SPANNING DOMAIN; CELL-SURFACE; GLYCOPROTEIN CONTAINS; CYTOPLASMIC DOMAIN; SECRETORY PATHWAY; RAT-LIVER; LOCALIZATION;
Keywords:
RETENTION; TYPE II PROTEIN; GOLGI APPARATUS; ENDOPLASMIC RETICULUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
B.L. Tang et al., "ENDOPLASMIC-RETICULUM RETENTION MEDIATED BY THE TRANSMEMBRANE DOMAIN OF TYPE-II MEMBRANE-PROTEINS SEC12P AND GLUCOSIDASE-1", European journal of cell biology, 73(2), 1997, pp. 98-104

Abstract

The yeast Sec12p, a type II protein localized to the yeast endoplasmic reticulum (ER), is similarly localized to the ER when expressed in mammalian cells. Replacing the transmembrane domain of the plasma membrane molecule dipeptidyl peptidase nl (D4) with that of Sec12p or the ER-localized enzyme glucosidase 1 resulted in the ER retention of the chimeric molecules, as assessed by immunocytochemical localization and the persistence of pulse-labeled proteins in the endoglycosidase H-sensitive form, Retention is not due to gross misfolding as these chimeras remained enzymatically active. Density gradient analysis revealed that the ER-localized chimeric molecules form high molecular weight oligomers quickly after synthesis. The type II transmembrane domain of ER proteins could therefore mediate retention in the ER.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 06:46:38