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Titolo:
Construction of recombinants Pseudomonas putida BO14 and Escherichia coli QEFCA8 for ferulic acid biotransformation to vanillin
Autore:
Okeke, BC; Venturi, V;
Indirizzi:
Int Ctr Genet Engn & Biotechnol, Bacteriol Unit, I-34012 Trieste, Italy Int Ctr Genet Engn & Biotechnol Trieste Italy I-34012 012 Trieste, Italy
Titolo Testata:
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
fascicolo: 1, volume: 88, anno: 1999,
pagine: 103 - 106
SICI:
1389-1723(199907)88:1<103:CORPPB>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLECULAR CHARACTERIZATION; GENES; TRANSFORMATIONS; BIOCONVERSION; FLUORESCENS; RHIZOBIUM; STRAIN; TRANS;
Keywords:
recombinants bacteria; biotransformation; ferulic acid; vanillin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Okeke, BC Gifu Univ, Dept Bioproc, Lab Microbial Engn & Technol, 1-1 Yanagido, Gifu 5011193, Japan Gifu Univ 1-1 Yanagido Gifu Japan 5011193 , Gifu 5011193, Japan
Citazione:
B.C. Okeke e V. Venturi, "Construction of recombinants Pseudomonas putida BO14 and Escherichia coli QEFCA8 for ferulic acid biotransformation to vanillin", J BIOSCI BI, 88(1), 1999, pp. 103-106

Abstract

Recombinants Pseudomonas putida BO14 and Escherichia coli QEFCA8 capable of ferulic acid biotransformation to vanillin mere constructed using homologous recombination and a PCR based cloning strategy, respectively. In the liquid culture of P. putida BO14, 26.81+/-2.30 mu g vanillin ml(-1) of culture filtrate was detected. In the case of recombinant E. coli QEFCA8, 19.37+/-1.95 mu g vanillin ml(-1) of culture filtrate was detected. Results indicate that the strains could be useful for the biotechnological production of vanillin, a very important flavoring substance.

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Documento generato il 02/04/20 alle ore 11:42:44