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Titolo:
Gonadotropin and steroid regulation of steroid receptor and aryl hydrocarbon receptor messenger ribonucleic acid in macaque granulosa cells during the periovulatory interval
Autore:
Chaffin, CL; Stouffer, RL; Duffy, DM;
Indirizzi:
Oregon Reg Primate Res Ctr, Div Reprod Sci, Beaverton, OR 97006 USA OregonReg Primate Res Ctr Beaverton OR USA 97006 Beaverton, OR 97006 USA Oregon Hlth Sci Univ, Dept Physiol & Pharmacol, Portland, OR 97201 USA Oregon Hlth Sci Univ Portland OR USA 97201 rmacol, Portland, OR 97201 USA
Titolo Testata:
ENDOCRINOLOGY
fascicolo: 10, volume: 140, anno: 1999,
pagine: 4753 - 4760
SICI:
0013-7227(199910)140:10<4753:GASROS>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRIMATE CORPUS-LUTEUM; HEPA 1C1C7 CELLS; PROGESTERONE-RECEPTOR; MENSTRUAL-CYCLE; ESTROGEN-RECEPTOR; ANDROGEN RECEPTOR; RHESUS-MONKEYS; RAT OVARY; GENE-EXPRESSION; RNA LEVELS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Stouffer, RL Oregon Reg Primate Res Ctr, Div Reprod Sci, 505 NW 185th Ave,Beaverton, OR 97006 USA Oregon Reg Primate Res Ctr 505 NW 185th Ave Beaverton OR USA 97006
Citazione:
C.L. Chaffin et al., "Gonadotropin and steroid regulation of steroid receptor and aryl hydrocarbon receptor messenger ribonucleic acid in macaque granulosa cells during the periovulatory interval", ENDOCRINOL, 140(10), 1999, pp. 4753-4760

Abstract

Although steroids play a local role(s) in ovulation and luteinization of the primate follicle, the dynamics of steroid receptor expression during the36- to 38-h periovulatory interval has yet to be elucidated. The present study examines the regulation of messenger RNAs (mRNAs) for progesterone (PR), androgen (AR), and estrogen (ER alpha, ER beta) receptors as well as thearyl hydrocarbon receptor (AhR) in macaque granulosa cells during controlled ovarian stimulation cycles before (0 h) and after (up to 36 h) administration of the ovulatory hCG bolus with or without steroid depletion and progestin replacement. All steroid receptor mRNAs were detected in granulosa cells before the ovulatory stimulus, as determined by RT-PCR. PR mRNA increased (P < 0.05) by 12 h after hCG; 24 and 36 h after hCG, levels were intermediate between 0-12 h. PR mRNA was reduced by steroid depletion throughout the periovulatory interval (P < 0.05); however, progestin replacement returned PR mRNA to control levels at 12 h. AR mRNA increased (P < 0.05) at 24 h post-hCG and remained at this level 36 h after hCG; steroid depletion did not alter AR mRNA levels. ER alpha mRNA did not change, whereas ER beta decreased 12-36 h after the ovulatory stimulus (P < 0.05). Steroid depletion reduced ER alpha mRNA 12 h after hCG, an effect partially reversible by progestin replacement, whereas ER beta mRNA was not affected by steroids. AhR mRNA was undetectable before the administration of hCG, but increased by 12 h(P < 0.05). These data demonstrate hCG-initiated, steroid-dependent (PR, ER alpha) and -independent (AR, ER beta, AhR) expression of receptor mRNAs in primate granulosa cells during the periovulatory interval. Differences inpatterns of expression may relate to diverse roles for steroid hormones and AhR ligands in periovulatory events.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 03:00:00