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Titolo:
Spleen autotransplantation provides restoration of functional splenic lymphoid compartments and improves the humoral immune response to pneumococcal polysaccharide vaccine
Autore:
Leemans, R; Harms, G; Rijkers, GT; Timens, W;
Indirizzi:
Univ Groningen Hosp, Dept Pathol, NL-9700 RB Groningen, Netherlands Univ Groningen Hosp Groningen Netherlands NL-9700 RB ningen, Netherlands Univ Groningen Hosp, Dept Surg, Med Ctr, NL-9700 RB Groningen, NetherlandsUniv Groningen Hosp Groningen Netherlands NL-9700 RB ningen, Netherlands Univ Hosp Children & Youth Wilhelmina Kinderzieke, Dept Immunol, Utrecht, Netherlands Univ Hosp Children & Youth Wilhelmina Kinderzieke Utrecht Netherlands s
Titolo Testata:
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
fascicolo: 3, volume: 117, anno: 1999,
pagine: 596 - 604
SICI:
0009-9104(199909)117:3<596:SAPROF>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
MARGINAL ZONE; AUTO-TRANSPLANTATION; ANTIBODY-RESPONSES; GERMINAL-CENTERS; TI-2 ANTIGENS; RAT; SPLENECTOMY; SUBPOPULATIONS; SEPSIS; SYSTEM;
Keywords:
autotransplantation; rat; pneumococcal vaccination; spleen; humoral immune response; ELISA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Timens, W Univ Groningen Hosp, Dept Pathol, POB 30-001, NL-9700 RB Groningen, Netherlands Univ Groningen Hosp POB 30-001 Groningen Netherlands NL-9700 RB
Citazione:
R. Leemans et al., "Spleen autotransplantation provides restoration of functional splenic lymphoid compartments and improves the humoral immune response to pneumococcal polysaccharide vaccine", CLIN EXP IM, 117(3), 1999, pp. 596-604

Abstract

After splenectomy, patients have an increased risk of overwhelming post-splenectomy infection (OPSI) or sepsis involving encapsulated bacteria such as pneumococs. The value of spleen autotransplantation after splenectomy because of trauma has long been questioned. Much attention has been given to the restoration of mononuclear phagocyte system (MPS) function, which appeared to be similar to that of splenectomized individuals. The presence of specific anti-pneumococcal antibodies may enhance phagocytosis of opsonized bacteria by other parts of the MPS, as present in the liver. Therefore, in the present study we have evaluated the restoration of the humoral immune response after spleen autotransplantation, especially to pneumococcal capsularpolysaccharides (PPS). Wistar rats were divided into three groups which were operated as follows:splenectomy, splenectomy followed by autotransplantation, and sham operation. After 12 weeks the rats were vaccinated with 23-valent pneumococcal vaccine. Blood samples were taken after 3 days, 3 and 6 weeks for anti-PPS IgM and IgG ELISA against types 3, 4, 6, 9, 14 and 23. In addition, immunohistological studies were performed on the autotransplants. Significant antibody titre rises were found in a main proportion of the autotransplanted rats, comparable to sham-operated rats. Splenectomized rats showed as well a significantly lower increase in immunoglobulin levels, as significant differences in the proportion of rats showing a minimum two-fold increase of antibody level, considered to represent an adequate response. The titres were highest 3 days after vaccination. Immunohistochemical studies demonstrated structurally functional autotransplants, including an intact marginal zone. Considering this significant antipneumococcal antibody response, spleen autotransplants can be expected to enable an improved humoral response to PPS, and to contribute to protection against OPSI after splenectomy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 18:24:08