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Titolo:
Viral load and CD69 molecule expression on freshly isolated and cultured mitogen-stimulated lymphocytes of children with perinatal HIV-1 infection
Autore:
de Martino, M; Rossi, ME; Azzari, C; Gelli, MG; Chiarelli, P; Galli, L; Vierucci, A;
Indirizzi:
Univ Chieti, Dept Med, Paediat Sect, I-66100 Chieti, Italy Univ Chieti Chieti Italy I-66100 ed, Paediat Sect, I-66100 Chieti, Italy Univ Florence, Dept Paediat, Florence, Italy Univ Florence Florence Italy iv Florence, Dept Paediat, Florence, Italy
Titolo Testata:
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
fascicolo: 3, volume: 117, anno: 1999,
pagine: 513 - 516
SICI:
0009-9104(199909)117:3<513:VLACME>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATION; CELLS; COMPLEX;
Keywords:
HIV-1; perinatal HIV-1 infection; HIV-1 load; CD69; activation molecules;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: de Martino, M Univ Chieti, Dept Med, Paediat Sect, Via Vestini, I-66100 Chieti, Italy Univ Chieti Via Vestini Chieti Italy I-66100 Chieti, Italy
Citazione:
M. de Martino et al., "Viral load and CD69 molecule expression on freshly isolated and cultured mitogen-stimulated lymphocytes of children with perinatal HIV-1 infection", CLIN EXP IM, 117(3), 1999, pp. 513-516

Abstract

HIV-1 in adults changes the proportion of mitogen-stimulated lymphocytes expressing the CD69 activation molecule, but little is known about this molecule expression on lymphocytes of HIV-1-infected (HIV-1(+)) children. Freshly isolated CD3(+), CD4(+), CD8(+) and CD19(+) and phytohaemagglutinin (PHA)-stimulated CD3(+), CD4(+) and CD8(+) lymphocytes co-expressing CD69 were investigated cross-sectionally (adopting a MoAb double-staining technique) in 24 HIV-1(+) children with severe disease and given anti-retroviral therapy and in 24 age-matched healthy children. CD69 results in HIV-1(+) children were correlated with plasma HIV-1 RNA load prospectively determined. HIV-1(+) compared with healthy children had higher frequencies of freshly isolated CD3(+)CD69(+) (2.4 +/- 2.2% versus 0.9 +/- 0.5%; P = 0.002) and CD8(+)CD69(+) (1.5 +/- 1.1% versus 0.5 +/- 0.2%; P < 0.0001) lymphocytes. The frequencies of CD4(+)CD69(+) and CD19(+)CD69(+) lymphocytes were similar. High viral load correlated with an elevated proportion of freshly isolated CD3(+)CD69(+) and CD8(+)CD69(+) lymphocytes. HIV-1(+) children showed reduced frequencies of PHA-stimulated CD3(+)CD69(+) (60.7 +/- 7.6% versus 86.1 +/- 7.6%; P<0.001), CD4(+)CD69(+) (73.6 +/- 18.2% versus 92.6 +/- 5.1%; P<0.001),and CD8(+)CD69(+) (51.0 +/- 19.1% versus 65.3 +/- 15.4%; P=0.007) lymphocytes. Virologic worsening within 6 months correlated with a low proportion of PHA-stimulated CD3(+)CD69(+) and CD8(+)CD69(+) lymphocytes. CD69 moleculeexpression reflected the coexistence of immune activation and immune deficiency in HIV-1 infection. Changes partly differed from those observed in HIV-1(+) adults. CD8(+)CD69(+) (but not CD4(+)CD69(+)) lymphocyte proportion correlated with virologic course, and an impaired ability of CD8(+) lymphocytes to express CD69 upon PHA stimulation preceded a virologic worsening.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/10/20 alle ore 04:46:26