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Titolo:
Signal transduction of thapsigargin-induced apoptosis in osteoblast
Autore:
Chae, HJ; Chae, SW; Weon, KH; Kang, JS; Kim, HR;
Indirizzi:
Wonkwang Univ, Dept Dent Pharmacol, Sch Dent, Iksan 570749, Chonbuk, SouthKorea Wonkwang Univ Iksan Chonbuk South Korea 570749 70749, Chonbuk, SouthKorea Wonkwang Univ, Inst Wonkwang Biomat Implant, Sch Dent, Chonbuk, South Korea Wonkwang Univ Chonbuk South Korea plant, Sch Dent, Chonbuk, South Korea Chonbuk Univ, Sch Med, Dept Pharmacol, Chonbuk, South Korea Chonbuk Univ Chonbuk South Korea , Dept Pharmacol, Chonbuk, South Korea
Titolo Testata:
BONE
fascicolo: 4, volume: 25, anno: 1999,
pagine: 453 - 458
SICI:
8756-3282(199910)25:4<453:STOTAI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
NF-KAPPA-B; MURINE CLONAL OSTEOBLASTS; DEATH GENE CED-3; CELL-DEATH; PROTEIN-KINASE; ENDOPLASMIC-RETICULUM; C-JUN; INTRACELLULAR CALCIUM; JNK ACTIVATION; MAP KINASES;
Keywords:
thapsigargin; osteoblast; apoptosis; caspase; JNK1; AP-1; NF-kappa B;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, HR Wonkwang Univ, Dept Dent Pharmacol, Sch Dent, Iksan 570749, Chonbuk, SouthKorea Wonkwang Univ Iksan Chonbuk South Korea 570749 honbuk, SouthKorea
Citazione:
H.J. Chae et al., "Signal transduction of thapsigargin-induced apoptosis in osteoblast", BONE, 25(4), 1999, pp. 453-458

Abstract

The toxicity of thapsigargin, a selective inhibitor of endoplasmic reticular Ca2+-ATPase, was investigated in osteoblasts. We induced apoptosis in murine osteoblastic MC3T3E1 cells by exposure to the thapsigargin. Thapsigargin transiently increased the phosphotransferase activity of c-Jun N-terminal kinases1 (JNK1), which might in turn activate transcriptional activity ofactivation protein-1 (AP-1). Wt? then prepared extracts from thapsigargin-treated MC3T3E1 cells and monitored cleavage of acetyl-YVAD-AMC and acetyl-DEVD-AMC, fluorogenic substrates for caspase 1-like and caspase 3-like proteases, respectively. Thapsigargin significantly increased the proteolytic activity of caspase 3-like proteases, but not the activity of caspase 1-likeproteases. Furthermore, thapsigargin increased the transcriptional activity of nuclear factor-kappa B (NF-kappa B), These data suggest that thapsigargin-induced apoptosis in osteoblasts may be via activation of JNK1, caspase3-like family proteases, and transcriptional factors including AP-1 and NF-kappa B. (C) 1999 by Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 15:10:48