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Titolo:
Microtubules are potential regulators of growth-plate chondrocyte differentiation and hypertrophy
Autore:
Farquharson, C; Lester, D; Seawright, E; Jefferies, D; Houston, B;
Indirizzi:
Roslin Inst, Bone Biol Grp, Roslin EH25 9PS, Midlothian, Scotland Roslin Inst Roslin Midlothian Scotland EH25 9PS 9PS, Midlothian, Scotland
Titolo Testata:
BONE
fascicolo: 4, volume: 25, anno: 1999,
pagine: 405 - 412
SICI:
8756-3282(199910)25:4<405:MAPROG>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONGITUDINAL BONE-GROWTH; EXTRACELLULAR-MATRIX; CELL-SHAPE; TIBIAL DYSCHONDROPLASIA; SIGNAL-TRANSDUCTION; GENE-EXPRESSION; DNA-SYNTHESIS; IN-VITRO; CARTILAGE; COLLAGEN;
Keywords:
tubulin; microtubules; chondrocytes; growth plate; hypertrophy; differential display;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Farquharson, C Roslin Inst, Bone Biol Grp, Roslin EH25 9PS, Midlothian, Scotland Roslin Inst Roslin Midlothian Scotland EH25 9PS , Scotland
Citazione:
C. Farquharson et al., "Microtubules are potential regulators of growth-plate chondrocyte differentiation and hypertrophy", BONE, 25(4), 1999, pp. 405-412

Abstract

Terminal differentiation of growth-plate chondrocytes is accompanied by the acquisition of a spherical morphology and a large increase in cell volume, These changes are likely to be associated with rearrangement of the cytoskeleton, but little information on this aspect of chondrocyte hypertrophy is available. We report a role for microtubules in the control of chondrocyte maturation and hypertrophy, Chick growth-plate chondrocytes were fractionated into five maturationally distinct populations hy Percoll density gradient centrifugation, and agarose gel differential display analysis was performed, We identified a 1200 bp cDNA fragment derived from a transcript that was most highly expressed in the hypertrophic chondrocytes, After cloning and sequencing, FASTA and BLAST analysis revealed 100% identity to chick beta(7)-tubulin. Differential expression was confirmed in a reverse transcription-polymerase chain reaction (RT-PCR) assay using specific primers for a 343 bp fragment from the 3' untranslated region of beta(7)-tubulin. beta(7)-tubulin was upregulated three-fold in fully hypertrophic chondrocytes compared with the other four fractions, which all had similar levels of expression, Immunocytochemical localization of beta-tubulin in chick growth-plate sections demonstrated little staining in the chondrocytes of the proliferating zone, but intense cytoplasmic staining was present in the large hypertrophic chondrocytes, In cell culture studies, the addition of colchicine (10(-6) mol/L) resulted in a higher rate of [H-3]-thymidine uptake (36.0%; p < 0.001), but lower amounts of alkaline phosphatase activity (69.1%; p < 0.001), collagen (49.1%; p < 0.01), and glycosaminoglycan (43.3%; p < 0.01) accumulation within the cell-matrix layer. Further evidence for the involvement of microtubules in chondrocyte differentiation and hypertrophy was obtained by morphological assessment of colchicine-treated growth-plate explant cultures, A partial failure of chondrocyte hypertrophy was observed, although collagen type X immunoreactivity was noted within the interstitial matrix. Further studies are required to identify the exact role of microtubules in chondrocyte hypertrophy, but the results presented here suggest that upregulation of P-tubulin may be required for increased microtubule synthesis during changes in cell size during the hypertrophic process. In addition, ascell-matrix interactions are required for chondrocyte maturation, microtubules may promote the differentiated phenotype as a result of their role in Golgi-mediated secretion of matrix proteins, (C) 1999 by Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 09:45:37