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Titolo:
Overexpression of phospholipase C-gamma 1 suppresses UVC-induced apoptosisthrough inhibition of c-fos accumulation and c-Jun N-terminal kinase activation in PC12 cells
Autore:
Lee, YH; Kim, SY; Kim, JR; Kim, KY; Kim, MJ; Ryu, SH; Suh, PG; Kim, JH;
Indirizzi:
Yeungnam Univ, Dept Biochem, Coll Med, Taegu 705717, South Korea Yeungnam Univ Taegu South Korea 705717 ll Med, Taegu 705717, South Korea Pohang Univ Sci & Technol, Dept Life Sci, Pohang 790784, South Korea Pohang Univ Sci & Technol Pohang South Korea 790784 790784, South Korea
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
fascicolo: 2-3, volume: 1440, anno: 1999,
pagine: 235 - 243
SICI:
1388-1981(19990922)1440:2-3<235:OOPC1S>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIGNAL-TRANSDUCTION; PROTEIN-KINASE; TYROSINE KINASE; THYMOCYTE APOPTOSIS; GENE-EXPRESSION; HA-RAS; RECEPTOR; DEATH; PHOSPHORYLATION; RADIATION;
Keywords:
ultraviolet-C; phospholipase C-gamma 1; apoptosis; c-Jun N-terminal kinase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, JH Yeungnam Univ, Dept Biochem, Coll Med, Taegu 705717, South Korea Yeungnam Univ Taegu South Korea 705717 Taegu 705717, South Korea
Citazione:
Y.H. Lee et al., "Overexpression of phospholipase C-gamma 1 suppresses UVC-induced apoptosisthrough inhibition of c-fos accumulation and c-Jun N-terminal kinase activation in PC12 cells", BBA-MOL C B, 1440(2-3), 1999, pp. 235-243

Abstract

Ultraviolet-C (UVC) irradiation induces DNA damage and UVC-irradiated cells undergo cell growth arrest to repair the damaged DNA or the induction of apoptosis to prevent the risk of neoplastic transformation. Phospholipase C-gamma 1 (PLC-gamma 1) is a mediator of growth factor induced-signal cascade, catalyzing the hydrolysis of phosphatidyl 4,5-bisphosphate to generate second messengers, diacylglycerol and inositol 1,4,5-trisphosphate (IP3) PLC-gamma 1 is activated by phosphorylation of tyrosine residues upon occupation of cell surface receptors by growth factors and plays an important role in controlling cellular proliferation and differentiation. In this study, we found that PLC-gamma 1 was tyrosine phosphorylated within 2.5 min after UVC irradiation. To investigate the role of UVC-induced tyrosine phosphorylation of PLC-gamma 1, we compared the effect of UVC between PLC-gamma 1 overexpressing cells and empty vector transfected cells. Overexpression of PLC-gamma 1 inhibited UVC-induced sub-diploid peak and DNA fragmentation. Northern blot analysis revealed that UVC-induced c-fos mRNA accumulation was inhibited in PLC-gamma 1 overexpressing cells, while c-jun expression was not affected. In addition, UVC-induced activation of c-Jun N-terminal kinase (JNK) was significantly suppressed in PLC-gamma 1 overexpressing cells. Theseresults suggest that PLC gamma 1 may associate with the protective function against the UVC-induced cell death progression via the inhibition of accumulation of c-fos mRNA and the inhibition of JNK kinase activity. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 03:06:16