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Titolo:
Protective effects of heme oxygenase-1 against oxidant-induced injury in the cultured human tracheal epithelium
Autore:
Yamada, N; Yamaya, M; Okinaga, S; Lie, R; Suzuki, T; Nakayama, K; Takeda, A; Yamaguchi, T; Itoyama, Y; Sekizawa, K; Sasaki, H;
Indirizzi:
Tohoku Univ, Sch Med, Dept Geriatr Med & Neurol, Sendai, Miyagi 980, JapanTohoku Univ Sendai Miyagi Japan 980 d & Neurol, Sendai, Miyagi 980, Japan
Titolo Testata:
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
fascicolo: 3, volume: 21, anno: 1999,
pagine: 428 - 435
SICI:
1044-1549(199909)21:3<428:PEOHOA>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISOLATED RAT HEPATOCYTES; OXIDATIVE STRESS; BILIRUBIN; INDUCTION; FERRITIN; ENZYME; IRON; PERMEABILITY; ANTIOXIDANT; FIBROBLASTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Sasaki, H Tohoku Univ, Sch Med, Dept Geriatr Med, Aoba Ku, Seiryo Machi 1-1, Sendai,Miyagi 980, Japan Tohoku Univ Seiryo Machi 1-1 Sendai Miyagi Japan 980 980, Japan
Citazione:
N. Yamada et al., "Protective effects of heme oxygenase-1 against oxidant-induced injury in the cultured human tracheal epithelium", AM J RESP C, 21(3), 1999, pp. 428-435

Abstract

To examine whether increases in heme oxygenase (HO)-1 activity have protective effects on the oxidant-induced injury of airway epithelial cells, human tracheal epithelial cells were cultured on a porous filter membrane, and electrical conductance (G) and mannitol flux across epithelial membrane were measured with Ussing's chamber methods and D-[H-3]mannitol, respectively. Hydrogen peroxide (H2O2; 1 mM) increased G with time from the baseline value of 6.0 +/- 0.6 to 17.8 +/- 0.9 mS/cm(2) at 6 h after administration (P <0.001). Likewise, H2O2 significantly increased mannitol flux through the cultured epithelium (P < 0.01). Pretreatment of cultured epithelial cells with hemin (10 mu M; 8 h) or interleukin (IL)-1 beta (10 ng/ml; 16 h) completely inhibited increases in G and mannitol flux induced by H2O2 Tin protoporphyrin IX (50 mu m) and zinc protoporphyrin TX (10 mu M), inhibitors of HO-1, reduced hemin-induced and IL-1 beta-induced inhibitory effects. Hemin treatment increased HO-1 messenger RNA expression, HO-1 protein production, and HO activity and bilirubin content as well as ferritin content in the cultured epithelial cells. Pretreatment with hemin and desferoxamine, which, like ferritin, can bind iron, inhibited H2O2-induced increases in G and mannitol permeability. Although exogenous bilirubin mimicked hemin-induced inhibitory effects, exogenous apoferritin failed to inhibit H2O2-induced effects on G and mannitol permeability. These findings suggest that HO-I induction provides protection against H2O2-induced injury of the cultured human airway epithelial cells in part via the HO-bilirubin pathway.

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Documento generato il 11/07/20 alle ore 10:36:19