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Titolo:
Crystallization and preliminary X-ray diffraction studies of monomeric isocitrate dehydrogenase from Corynebacterium glutamicum
Autore:
Audette, GF; Quail, JW; Hayakawa, K; Bai, C; Chen, RD; Delbaere, LTJ;
Indirizzi:
Univ Saskatchewan, Dept Biochem, Saskatoon, SK S7K 5E5, Canada Univ Saskatchewan Saskatoon SK Canada S7K 5E5 skatoon, SK S7K 5E5, Canada Univ Saskatchewan, Dept Chem, Saskatoon, SK S7K 5C9, Canada Univ Saskatchewan Saskatoon SK Canada S7K 5C9 skatoon, SK S7K 5C9, Canada
Titolo Testata:
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
, volume: 55, anno: 1999,
parte:, 9
pagine: 1584 - 1585
SICI:
0907-4449(199909)55:<1584:CAPXDS>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENZYME; PURIFICATION; SPECIFICITY; BACTERIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Audette, GF Univ Saskatchewan, Dept Biochem, 107 Wiggins Rd, Saskatoon, SKS7K 5E5, Canada Univ Saskatchewan 107 Wiggins Rd Saskatoon SK Canada S7K 5E5 a
Citazione:
G.F. Audette et al., "Crystallization and preliminary X-ray diffraction studies of monomeric isocitrate dehydrogenase from Corynebacterium glutamicum", ACT CRYST D, 55, 1999, pp. 1584-1585

Abstract

A monomeric isocitrate dehydrogenase has been crystallized for the first time. This enzyme catalyzes the conversion of isocitrate to oxalosuccinate and subsequently to alpha-ketoglutarate and CO2; the coenzyme NADP(+) is reduced to NADPH during the reaction. Polyethylene glycol 2000 monomethyl ether was used to crystallize the enzyme in space group CZ with unit-cell parameters a = 137.1, b = 54.6, c = 126.4 Angstrom beta = 108.2 degrees. The very small crystal (0.05 x 0.20 x 0.05 mm) diffracted to 3.5 Angstrom d spacing using synchrotron radiation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 22:10:25