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Titolo:
CELLULAR-LOCALIZATION OF INDUCIBLE NITRIC-OXIDE SYNTHASE IN EXPERIMENTAL ENDOTOXIC-SHOCK IN THE RAT
Autore:
COOK HT; BUNE AJ; JANSEN AS; TAYLOR GM; LOI RK; CATTELL V;
Indirizzi:
ST MARYS HOSP,SCH MED,IMPERIAL COLL SCI TECHNOL & MED,DEPT HISTOPATHOL,NORFOLK PL LONDON W2 1PG ENGLAND ST MARYS HOSP,SCH MED,IMPERIAL COLL SCI TECHNOL & MED,DEPT HISTOPATHOL LONDON W2 1PG ENGLAND
Titolo Testata:
Clinical science
fascicolo: 2, volume: 87, anno: 1994,
pagine: 179 - 186
SICI:
0143-5221(1994)87:2<179:COINSI>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
L-ARGININE; INSITU HYBRIDIZATION; HEPATIC-INJURY; INDUCTION; HEPATOCYTES; INHIBITION; NITRATE;
Keywords:
ENDOTOXIN; LIVER; MACROPHAGES; MONOCYTES; NITRIC OXIDE SYNTHASE; SEPTIC SHOCK; SPLEEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
H.T. Cook et al., "CELLULAR-LOCALIZATION OF INDUCIBLE NITRIC-OXIDE SYNTHASE IN EXPERIMENTAL ENDOTOXIC-SHOCK IN THE RAT", Clinical science, 87(2), 1994, pp. 179-186

Abstract

1. Endotoxin induces a shock-like syndrome with increased nitric oxide synthesis. To clarify the cellular source of NO in endotoxic shock we used immunohistochemistry and in situ hybridization to localize inducible NO synthase in rats given Corynebacterium parvum polysaccharide or lipopolysaccharide. Immunohistochemistry was carried out with an antibody raised against a synthetic peptide of mouse macrophage NO synthase. In situ hybridization was performed with S-35-labelled oligonucleotide probes corresponding to cDNA sequences common to mouse macrophage inducible NO synthase and rat vascular smooth inducible NO synthase. Monocytes and macrophages were identified by immunohistochemistry with the mouse monoclonal antibody ED1. 2. After lipopolysaccharide alone, the major site of NO synthase induction was monocytes and macrophages in multiple organs, principally liver and spleen. Branchial, bile duct, intestinal and bladder epithelium and some hepatocytes also expressed inducible NO synthase. Expression peaked at 5h and had returned tonormal by 12h except in spleen. 3. After priming with C. parvum, lipopolysaccharide led to a similar distribution of inducible NO synthase as lipopolysaccharide alone, but in addition there was more prominent hepatocyte staining, staining in macrophage granulomas in the liver and inducible NO synthase was present in some endothelial cells in the aorta. 4. These findings provide a direct demonstration of the cellularlocalization of inducible NO synthase after lipopolysaccharide.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 11:28:56