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Titolo:
Colon cancer: p53 expression and DNA ploidy. Their relation to proximal ordistal tumor site
Autore:
Garcia, JMGH; Berenguel, AB; Recio, LV; Moreno, AJM; Garrido, JR; Vicente, AM;
Indirizzi:
Univ Malaga, Sch Med, Dept Pathol, Malaga, Spain Univ Malaga Malaga Spain iv Malaga, Sch Med, Dept Pathol, Malaga, Spain
Titolo Testata:
REVISTA ESPANOLA DE ENFERMEDADES DIGESTIVAS
fascicolo: 7, volume: 91, anno: 1999,
pagine: 485 - 488
SICI:
1130-0108(199907)91:7<485:CCPEAD>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLORECTAL-CANCER; MICROSATELLITE INSTABILITY; ALLELIC LOSS; CARCINOMA; GENE; MUTATIONS; ASSOCIATION; CYTOMETRY; SURVIVAL; RECTUM;
Keywords:
colon cancer; p53 expression; DNA ploidy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Garcia, JMGH Fac Med, Dept Anat Patol, Campus Univ S-N, Malaga 29071, Spain Fac Med Campus Univ S-N Malaga Spain 29071 laga 29071, Spain
Citazione:
J.M.G.H. Garcia et al., "Colon cancer: p53 expression and DNA ploidy. Their relation to proximal ordistal tumor site", REV ESP E D, 91(7), 1999, pp. 485-488

Abstract

Overexpression of nuclear p53 and DNA ploidy were analyzed in a series of 65 colorectal adenocarcinomas and correlated with standard clinical and pathological variables (Dukes stage, tumor site, histological grade and type, and nature of the tumor margins). Immunohistochemical tests were done with the DO-7 monoclonal antibody, using formalin-fixed tissue samples and an antigen retrieval solution. Levels of p53 expression were evaluated using a semiquantitative grading system (CAS 200, BD). Nuclear staining of more than 15% of neoplastic cells was observed in 35 samples (53.8%), which were classified as p53-positive. DNA content was measured by flow cytometry in samples of fresh tissue. Tumor site had a significant direct relationship with DNA ploidy (p < 0.01) and p53 expression (p < 0.001). Proximal tumors were more frequently diploid than were distal tumors (78.6% vs 32%). Moreover, distal neoplasms showed more p53 expression than proximal tumors (64.6% vs 14.3%). However, there was no correlation between the other clinical or pathological variables and the pathological parameter p53 expression and DNA ploidy. Our data support the hypothesis that mechanisms of colorectal carcinogenesis may differ in proximal and distal neoplasms.

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Documento generato il 03/04/20 alle ore 13:03:05