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Titolo:
ISOLATION AND CHARACTERIZATION OF A NONPIGMENTED VARIANT OF PORPHYROMONAS-ENDODONTALIS
Autore:
SUZUKI K; IKEDA T; NAKAMURA H; YOSHIMURA F;
Indirizzi:
AICHI GAKUIN UNIV,SCH DENT,DEPT MICROBIOL,CHIKUSA KU,1-100 KUSUMOTO CHO NAGOYA AICHI 464 JAPAN AICHI GAKUIN UNIV,SCH DENT,DEPT MICROBIOL,CHIKUSA KU NAGOYA AICHI 464JAPAN AICHI GAKUIN UNIV,SCH DENT,DEPT ENDODONT NAGOYA AICHI 464 JAPAN
Titolo Testata:
Oral microbiology and immunology
fascicolo: 3, volume: 12, anno: 1997,
pagine: 155 - 161
SICI:
0902-0055(1997)12:3<155:IACOAN>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLACK-PIGMENTED BACTEROIDES; TRYPSIN-LIKE-ENZYME; GINGIVALIS W50; EXTRACELLULAR VESICLES; DNA; INFECTIONS; BACTEROIDES-GINGIVALIS-W50; PERIODONTITIS; PREVALENCE; VIRULENCE;
Keywords:
PORPHYROMONAS ENDODONTALIS; NONPIGMENTED VARIANT; DNA-DNA HYBRIDIZATION; VANCOMYCIN RESISTANCE; OUTER MEMBRANE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
K. Suzuki et al., "ISOLATION AND CHARACTERIZATION OF A NONPIGMENTED VARIANT OF PORPHYROMONAS-ENDODONTALIS", Oral microbiology and immunology, 12(3), 1997, pp. 155-161

Abstract

Porphyromonas endodontalis forms dark colonies on media containing blood. We isolated, from an infected root canal, a non-black-pigmented P. endodontalis variant, KSEW01, which forms beige colonies on blood agar media. To characterize this variant, we compared its properties with those of two black-pigmented P. endodontalis strains, ATCC35406 and KSE105. Strain KSEW01 had a gelatinase activity comparable to that of the pigmented strains. Cell lysates of these three strains resolved bySDS-PAGE electrophoresis showed similar protein patterns. Quantitative DNA-DNA hybridization experiments indicated high homology be tween the nonpigmented strain KSEW01 and the two dark-pigmented strains. Fromthese results, we identified strain KSEW01 as a P. endodontalis nonpigmented variant. DNA restriction endonuclease analysis indicated that the variant was closely related to a pigmented strain, KSE105. In constrast to the pigmented strains, strain KSEW01 did not degrade hemoglobin and formed no vesicles when cultured in the presence of blood. The susceptibilities of these three strains to 22 antibiotics were similarexcept for vancomycin. The nonpigmented variant was the most resistant to vancomycin (MIC: ATCC35406, 6.25 mu g/ml; KSE105, 12.5 mu g/ml; KSEW01, 100 mu g/ml). Overall, a relationship may exist between the presence of black-pigmentation and outer membrane systems of P. endodontalis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 23:53:15