Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Assignment of muscarinic receptor subtypes mediating G-protein modulation of Ca2+ channels by using knockout mice
Autore:
Shapiro, MS; Loose, MD; Hamilton, SE; Nathanson, NM; Gomeza, J; Wess, J; Hille, B;
Indirizzi:
Univ Washington, Sch Med, Dept Physiol & Biophys, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 iol & Biophys, Seattle, WA 98195 USA Univ Washington, Sch Med, Dept Pharmacol, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 ept Pharmacol, Seattle, WA 98195 USA Oberlin Coll, Neurosci Program, Oberlin, OH 44074 USA Oberlin Coll Oberlin OH USA 44074 Neurosci Program, Oberlin, OH 44074 USA NIDDKD, Bioorgan Chem Lab, NIH, Bethesda, MD 20892 USA NIDDKD Bethesda MDUSA 20892 oorgan Chem Lab, NIH, Bethesda, MD 20892 USA
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 19, volume: 96, anno: 1999,
pagine: 10899 - 10904
SICI:
0027-8424(19990914)96:19<10899:AOMRSM>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT SYMPATHETIC NEURONS; BETA-GAMMA-SUBUNITS; CALCIUM CHANNELS; N-TYPE; PERIPHERAL NEURONS; TARGETED DISRUPTION; VOLTAGE DEPENDENCE; CURRENT INHIBITION; GANGLION NEURONS; K+ CURRENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Hille, B Univ Washington, Sch Med, Dept Physiol & Biophys, Box 357290, Seattle, WA 98195 USA Univ Washington Box 357290 Seattle WA USA 98195 tle, WA 98195 USA
Citazione:
M.S. Shapiro et al., "Assignment of muscarinic receptor subtypes mediating G-protein modulation of Ca2+ channels by using knockout mice", P NAS US, 96(19), 1999, pp. 10899-10904

Abstract

There are five known subtypes of muscarinic receptors (M-1-M-5) We have used knockout mice lacking the M-1, M-2, or M-4 receptors to determine which subtypes mediate modulation of voltage-gated Ca2+ channels in mouse sympathetic neurons. Muscarinic agonists modulate N- and L-type Ca2+ channels in these neurons through two distinct G-protein-mediated mechanisms. One pathway is fast and membrane-delimited and inhibits N- and P/Q-type channels by shifting their activation to more depolarized potentials. The other is slow and voltage-independent and uses a diffusible cytoplasmic messenger to inhibit both Ca2+ channel types. Using patch-clamp methods on acutely dissociated sympathetic neurons, we isolated each pathway by pharmacological and kinetic means and found that each one is nearly absent in a particular knockout mouse. The fast and voltage-dependent pathway is lacking in the M-2 receptor knockout mice; the slow and voltage-independent pathway is absent from the M-1 receptor knockout mice; and neither pathway is affected in the Mg receptor knockout mice. The knockout effects are clean and are apparently not accompanied by compensatory changes in other muscarinic receptors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 21:35:09