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Titolo:
Tobacco O-methyltransferases involved in phenylpropanoid metabolism. The different caffeoyl-coenzyme A/5-hydroxyferuloyl-coenzyme A 3/5-O-methyltransferase and caffeic acid/5-hydroxyferulic acid 3/5-O-methyltransferase classes have distinct substrate specificities and expression patterns
Autore:
Maury, S; Geoffroy, P; Legrand, M;
Indirizzi:
Univ Strasbourg 1, CNRS, Inst Biol Mol Plantes, F-67084 Strasbourg, FranceUniv Strasbourg 1 Strasbourg France F-67084 , F-67084 Strasbourg, France
Titolo Testata:
PLANT PHYSIOLOGY
fascicolo: 1, volume: 121, anno: 1999,
pagine: 215 - 223
SICI:
0032-0889(199909)121:1<215:TOIIPM>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-SUSPENSION CULTURES; ELICITOR-MEDIATED RESPONSES; ADENOSYL-L-METHIONINE; LIGNIN BIOSYNTHESIS; A 3-O-METHYLTRANSFERASE; MOLECULAR-CLONING; MOSAIC-VIRUS; FUNGAL ELICITOR; 4-COUMARATE-COENZYME-A LIGASE; COA 3-O-METHYLTRANSFERASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
62
Recensione:
Indirizzi per estratti:
Indirizzo: Legrand, M Univ Strasbourg 1, CNRS, Inst Biol Mol Plantes, 12 Rue Gen Zimmer, F-67084Strasbourg, France Univ Strasbourg 1 12 Rue Gen Zimmer Strasbourg France F-67084
Citazione:
S. Maury et al., "Tobacco O-methyltransferases involved in phenylpropanoid metabolism. The different caffeoyl-coenzyme A/5-hydroxyferuloyl-coenzyme A 3/5-O-methyltransferase and caffeic acid/5-hydroxyferulic acid 3/5-O-methyltransferase classes have distinct substrate specificities and expression patterns", PLANT PHYSL, 121(1), 1999, pp. 215-223

Abstract

The biosynthesis of lignin monomers involves two methylation steps catalyzed by orthodiphenol-O-methyltransferases: caffeic acid/5-hydroxyferulic acid 3/5-O-methyltransferases (COMTs) and caffeoyl-coenzyme A (CoA)/5-hydroxyferuloyl-CoA 3/5-O-methyltransferases (CCoAOMTs). Two COMT classes (I and II) were already known to occur in tobacco (Nicotiana tabacum) and three distinct CCoAOMT classes have now been characterized. These three CCoAOMT classes displayed a maximum level of expression at different stages of stem development, in accordance with their involvement in the synthesis of lignin guaiacyl units. Expression profiles upon tobacco mosaic virus infection of tobacco leaves revealed a biphasic pattern of induction for COMT I, COMT II, and CCoAOMTs. The different isoforms were expressed in Escherichia coli andour results showed that CCoAOMTs and, more surprisingly, COMTs efficientlymethylated hydroxycinnamoyl-CoA esters. COMT I was also active toward 5-hydroxyconiferyl alcohol, indicating that COMT I that catalyzes syringyl unitsynthesis in planta may operate at the free acid, CoA ester, or alcohol levels. COMT II that is highly inducible by infection also accepted caffeoyl-CoA as a substrate, thus suggesting a role in ferulate derivative deposition in the walls of infected cells. Tobacco appears to possess an array of O-methyltransferase isoforms with variable efficiency toward the diverse plant o-diphenolic substrates.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/04/20 alle ore 02:03:03