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Titolo:
THE FIRST 5 AMINO-ACIDS OF THE CARBOXYL-TERMINUS OF PHOSPHATIDYLINOSITOL TRANSFER PROTEIN (PITP)-ALPHA PLAY A CRITICAL ROLE IN INOSITOL LIPID SIGNALING - TRANSFER ACTIVITY OF PITP IS ESSENTIAL BUT NOT SUFFICIENT FOR RESTORATION OF PHOSPHOLIPASE-C SIGNALING
Autore:
HARA S; SWIGART P; JONES D; COCKCROFT S;
Indirizzi:
UNIV LONDON UNIV COLL,DEPT PHYSIOL,UNIV ST,ROCKEFELLER BLDG LONDON WC1E 6JJ ENGLAND UNIV LONDON UNIV COLL,DEPT PHYSIOL LONDON WC1E 6JJ ENGLAND
Titolo Testata:
The Journal of biological chemistry
fascicolo: 23, volume: 272, anno: 1997,
pagine: 14908 - 14913
SICI:
0021-9258(1997)272:23<14908:TF5AOT>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
BOVINE BRAIN; CA2+-ACTIVATED SECRETION; SPECIFICITY; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
S. Hara et al., "THE FIRST 5 AMINO-ACIDS OF THE CARBOXYL-TERMINUS OF PHOSPHATIDYLINOSITOL TRANSFER PROTEIN (PITP)-ALPHA PLAY A CRITICAL ROLE IN INOSITOL LIPID SIGNALING - TRANSFER ACTIVITY OF PITP IS ESSENTIAL BUT NOT SUFFICIENT FOR RESTORATION OF PHOSPHOLIPASE-C SIGNALING", The Journal of biological chemistry, 272(23), 1997, pp. 14908-14913

Abstract

Phosphatidylinositol transfer protein (PITP) is essential for phospholipase C signaling and for constitutive and regulated vesicular traffic. PITP has a single lipid-binding site that can reversibly bind phosphatidylinositol (PI) and phosphatidylcholine (PC) and transfer these lipids between membrane compartments in vitro. The role of the carboxylterminus was examined by comparing wild-type PITP alpha with PITP alpha in which 5, 10, and 20 amino acids were deleted from the C terminus. Delta 5- and Delta 10-PITP had reduced PI and PC transfer activitiescompared with wild-type PITP, with the effect on PI transfer being more marked than that on PC transfer. Delta 20-PITP was inactive at all concentrations tested. All three truncated mutants were unable to restore G-protein-mediated phospholipase C beta stimulation in HL-60 cells. Delta 5- and Delta 10-PITP, but not Delta 20-PITP, inhibited the signaling function of wild-type protein without any effect on lipid transfer in vitro. We conclude that (a) the carboxyl terminus of PITP playsa critical role in phospholipase C signaling; (b) the transfer activity is not the only determining factor that dictates the restorative function of PITP in inositol lipid signaling; and (c) the dominant inhibitory effects of Delta 5- and Delta 10-PITP on wild-type PITP in phospholipase C signaling suggest the existence of a receptor for PITP.

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Documento generato il 14/07/20 alle ore 12:39:02