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Titolo:
Characterization of neutralizing anti-pre-S1 and anti-pre-S2 (HBV) monoclonal antibodies and their fragments
Autore:
Kuttner, G; Kramer, A; Schmidtke, G; Giessmann, E; Dong, L; Roggenbuck, D; Scholz, C; Seifert, M; Stigler, RD; Schneider-Mergener, J; Porstmann, T; Hohne, W;
Indirizzi:
Humboldt Univ, Univ Klinikum Charite, Inst Biochem, D-10098 Berlin, Germany Humboldt Univ Berlin Germany D-10098 st Biochem, D-10098 Berlin, Germany Humboldt Univ, Univ Klinikum Charite, Inst Med Immunol, D-10098 Berlin, Germany Humboldt Univ Berlin Germany D-10098 ed Immunol, D-10098 Berlin, Germany
Titolo Testata:
MOLECULAR IMMUNOLOGY
fascicolo: 10, volume: 36, anno: 1999,
pagine: 669 - 683
SICI:
0161-5890(199907)36:10<669:CONAAA>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEPATITIS-B VIRUS; LIVER PLASMA-MEMBRANES; SURFACE-ANTIGEN; HYPERVARIABLE REGIONS; SYNTHETIC PEPTIDES; PRES2 REGION; PROTEIN; EPITOPE; BINDING; PHAGE;
Keywords:
monoclonal antibody; Fab; scFv; hepatitis B; pre-S1; pre-S2; antigen specificity; epitope mapping; peptide library; substitutional analysis; flexible docking;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Kuttner, G Humboldt Univ, Univ Klinikum Charite, Inst Biochem, Schumannstr20-21, D-10098 Berlin, Germany Humboldt Univ Schumannstr 20-21 Berlin Germany D-10098 Germany
Citazione:
G. Kuttner et al., "Characterization of neutralizing anti-pre-S1 and anti-pre-S2 (HBV) monoclonal antibodies and their fragments", MOL IMMUNOL, 36(10), 1999, pp. 669-683

Abstract

Single-chain Fv fragments (scFv) were generated from two murine monoclonalantibodies directed to the neutralizing epitopes of the pre-Si and pre-SZ region of hepatitis B virus, respectively, using different assembly cloningstrategies. The scFv fragments were solubly expressed in E, coli. Dissociation constants were in the nanomolar range for all forms (whole IgG antibodies, Fab fragment and scFv fragments), The epitopes of both antibodies weremapped using solid phase peptide synthesis on continuous cellulose membranes and turned out to be linear determinants. The minimal epitope for the anti-pre-S2 antibody 1F6 was identified to be DPRVRGLYF (amino acid 133-141 of the pre-S region). For the anti-pre-Si antibody MA 18/7 the minimal epitope proved to be the hexamer LDPAFR (amino acid 30-35 of the pre-S region), Complete substitutional analyses as well as truncation experiments revealedkey residues for these antibody-antigen interactions. On the basis of those results we used computer-assisted modeling techniques to suggest models for both antibody-peptide interactions providing insight into the structuralbasis of these molecular recognitions. (C) 1999 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 09:07:20