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Titolo:
New Bet v 1 isoforms including a naturally occurring truncated form of theprotein derived from Austrian birch pollen
Autore:
Friedl-Hajek, R; Radauer, C; ORiordain, G; Hoffmann-Sommergruber, K; Leberl, K; Scheiner, O; Breiteneder, H;
Indirizzi:
Univ Vienna, Dept Gen & Expt Pathol, AKH EBO 3Q, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 ol, AKH EBO 3Q, A-1090 Vienna, Austria
Titolo Testata:
MOLECULAR IMMUNOLOGY
fascicolo: 10, volume: 36, anno: 1999,
pagine: 639 - 645
SICI:
0161-5890(199907)36:10<639:NBV1II>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-CELL EPITOPES; X-RAY; ALLERGEN; BET-V-1; PURIFICATION; IMMUNOTHERAPY; REACTIVITY; ANTIBODIES; IGE;
Keywords:
type I allergy; Bet v 1; isoforms; IgE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Radauer, C Univ Vienna, Dept Gen & Expt Pathol, AKH EBO 3Q, Waehringer Guertel 18-20,A-1090 Vienna, Austria Univ Vienna Waehringer Guertel 18-20 Vienna Austria A-1090 ria
Citazione:
R. Friedl-Hajek et al., "New Bet v 1 isoforms including a naturally occurring truncated form of theprotein derived from Austrian birch pollen", MOL IMMUNOL, 36(10), 1999, pp. 639-645

Abstract

Bet v 1, the major pollen allergen from white birch, displays a considerable degree of heterogeneity. Until now, all molecular and immunological characterization studies of Bet v 1 isoforms have been performed with commercially available pollen of Swedish origin. In regard to clinical studies with Austrian birch pollen allergic individuals, knowledge about the isoform repertoire in Austrian birch pollen was necessary, cDh'As coding for Bet v 1 isoforms from Austrian birch pollen were cloned by PCR amplification and sequenced. Besides the Austrian variants of the Swedish isoforms Bet v la (62%of the clones), ALK167 (4%), and Bet v 1d/h, Bet v 1g, and Bet v 11 (24%),three sequences with a significantly lower homology to known isoforms and two Bet v 1a-homologous sequences with a 7 bp insertion coding for a truncated protein were detected. No Austrian variants of the majority of the Swedish isoforms were found. The isoforms coding for truncated proteins were expressed in Escherichia coli and tested by immunoblotting. They bound a polyclonal anti-Bet v 1 antibody but did not recognize birch pollen allergic patients' serum IgE and two Bet v 1-specific monoclonal antibodies. The similarity of the Bet v 1 isoform patterns of Swedish and Austrian birch pollen justifies the use of Bet v 1 derived from Swedish pollen for clinical studies with birch pollen allergic individuals from outside Northern Europe. (C)1999 Elsevier Science Ltd. All rights reserved.

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Documento generato il 19/07/18 alle ore 13:27:18