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Titolo:
Assessment of striatal D1 and D2 dopamine receptor-G protein coupling by agonist-induced [S-35]GTP gamma S binding
Autore:
Geurts, M; Hermans, E; Maloteaux, JM;
Indirizzi:
Univ Catholique Louvain, Pharmacol Lab, FARL 5410, B-1200 Brussels, Belgium Univ Catholique Louvain Brussels Belgium B-1200 B-1200 Brussels, Belgium
Titolo Testata:
LIFE SCIENCES
fascicolo: 16, volume: 65, anno: 1999,
pagine: 1633 - 1645
SICI:
0024-3205(19990910)65:16<1633:AOSDAD>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAMMA-S BINDING; GUANINE-NUCLEOTIDE-BINDING; HIGH-AFFINITY GTPASE; RAT CEREBRAL-CORTEX; ADENYLATE-CYCLASE; BRAIN MEMBRANES; FUNCTIONAL-ACTIVITY; INDUCED ACTIVATION; ERGOT DERIVATIVES; LIGAND-BINDING;
Keywords:
[S-35]GTP gamma S binding; spiperone; striatal membranes; G protein; D1 dopamine receptor; D2 dopamine receptor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Hermans, E Univ Catholique Louvain, Pharmacol Lab, FARL 5410, Ave Hippocrate 54, B-1200 Brussels, Belgium Univ Catholique Louvain Ave Hippocrate 54 Brussels Belgium B-1200
Citazione:
M. Geurts et al., "Assessment of striatal D1 and D2 dopamine receptor-G protein coupling by agonist-induced [S-35]GTP gamma S binding", LIFE SCI, 65(16), 1999, pp. 1633-1645

Abstract

The dopamine receptor-mediated modulation of guanosine 5'-O-(gamma[S-35]thio)triphosphate ([S-35]GTP gamma S) binding has been characterized in rat striatal membranes. In optimized experimental conditions, the potency of dopamine was 4.47 mu M [3.02 - 6.61 mu M] and a maximal response representing 54.8 +/- 4.5 % increase above basal level was observed. Data obtained with different agonists and antagonists clearly revealed that the most importantfraction of this response was reflecting D2 receptor activation. Further analysis with specific antagonists also supported evidence for the involvement of Dl dopamine receptors. The potencies of compounds interacting with D1and D2 receptors were deduced from [S-35]GTP gamma S binding experiments and compared with their binding affinities for these receptors measured in similar experimental conditions. A good correlation between these parameterswas observed, supporting the applicability of this technique for the studyof dopamine receptors in the central nervous system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/06/20 alle ore 01:51:45