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Titolo:
Stoichiometry of monoclonal antibody neutralization of T-cell line-adaptedhuman immunodeficiency virus type 1
Autore:
Schonning, K; Lund, O; Lund, OS; Hansen, JES;
Indirizzi:
Hvidovre Hosp, Dept Clin Microbiol 445, DK-2650 Hvidovre, Denmark HvidovreHosp Hvidovre Denmark DK-2650 ol 445, DK-2650 Hvidovre, Denmark Hvidovre Hosp, Lab Infect Dis 144, DK-2650 Hvidovre, Denmark Hvidovre Hosp Hvidovre Denmark DK-2650 is 144, DK-2650 Hvidovre, Denmark
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 10, volume: 73, anno: 1999,
pagine: 8364 - 8370
SICI:
0022-538X(199910)73:10<8364:SOMANO>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENVELOPE GLYCOPROTEIN; HIV-1; GP120; POLIOVIRUS; KINETICS; OLIGOMER; IMMUNIZATION; SENSITIVITY; INFECTIVITY; ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Schonning, K Hvidovre Hosp, Dept Clin Microbiol 445, Kettegard Alle 30, DK-2650 Hvidovre, Denmark Hvidovre Hosp Kettegard Alle 30 Hvidovre Denmark DK-2650 ark
Citazione:
K. Schonning et al., "Stoichiometry of monoclonal antibody neutralization of T-cell line-adaptedhuman immunodeficiency virus type 1", J VIROLOGY, 73(10), 1999, pp. 8364-8370

Abstract

In order to study the stoichiometry of monoclonal antibody (MAb) neutralization of T-cell line-adapted human immunodeficiency virus type 1 (HIV-1) inantibody excess and under equilibrium conditions, we exploited the abilityof HIV-I to generate mixed oligomers when different env genes are coexpressed, By the coexpression of Env glycoproteins that either can or cannot bind a neutralizing MAb in an env transcomplementation assay, virions were generated in which the proportion of MAb binding sites could be regulated. As the proportion of MAb binding sites in Env chimeric virus increased, MAb neutralization gradually increased. Virus neutralization by virion aggregation was minimal, as MAb binding to HIV-1 Env did not interfere with an AMLV Env-mediated infection by HN-I(AMLV/HIV-1) pseudotypes of CD4(-) NEK293 cells. MAb neutralization of chimeric virions could be described as a third-order function of the proportion of Env antigen refractory to MAb binding. This scenario is consistent with the Env oligomer Constituting the minimal functional unit and neutralization occurring incrementally as each Env oligomer binds MAb. Alternatively, the data could be fit to a sigmoid function. Thus, these data could not exclude the existence of a threshold for neutralization. However, results from MAb neutralization of chimeric virus containing wild-type Env and Env defective in CD4 binding aas readily explained by amodel of incremental MAb neutralization. In summary, the data indicate that MAb neutralization of T-cell line-adapted HIV-1 is incremental rather than all or none and that each MAb binding an Env oligomer reduces the likelihood of infection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 23:38:05