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Titolo:
A chimeric type 2 adenovirus vector with a type 17 fiber enhances gene transfer to human airway epithelia
Autore:
Zabner, J; Chillon, M; Grunst, T; Moninger, TO; Davidson, BL; Gregory, R; Armentano, D;
Indirizzi:
Univ Iowa, Coll Med, Dept Internal Med, Iowa City, IA 52242 USA Univ IowaIowa City IA USA 52242 pt Internal Med, Iowa City, IA 52242 USA Genzyme Corp, Dept Gene Therapy, Framingham, MA 01709 USA Genzyme Corp Framingham MA USA 01709 ne Therapy, Framingham, MA 01709 USA
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 10, volume: 73, anno: 1999,
pagine: 8689 - 8695
SICI:
0022-538X(199910)73:10<8689:ACT2AV>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
VIRUS-RECEPTOR INTERACTION; ALPHA-V INTEGRINS; CYSTIC-FIBROSIS; IN-VITRO; TRACHEAL EPITHELIUM; COOPERATIVE BINDING; NASAL EPITHELIUM; PENTON BASE; HELA-CELLS; TROPISM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Zabner, J Univ Iowa, Coll Med, Dept Internal Med, 500 EMRB, Iowa City, IA 52242 USA Univ Iowa 500 EMRB Iowa City IA USA 52242 owa City, IA 52242 USA
Citazione:
J. Zabner et al., "A chimeric type 2 adenovirus vector with a type 17 fiber enhances gene transfer to human airway epithelia", J VIROLOGY, 73(10), 1999, pp. 8689-8695

Abstract

In studies of the genetic disease cystic fibrosis, recombinant adenovirus type 2 (Ad2) and Ad5 are being investigated as vectors to transfer cystic fibrosis transmembrane conductance regulator cDNA to airway epithelia. However, earlier work has shown that human airway epithelia are resistant to infection by Ad2 and Ad5. Therefore, we examined the efficiency of other adenovirus serotypes at infecting airway epithelia. We found that several serotypes of adenoviruses, in particular, wild-type Ad17, infected a greater number of cells than wild-type Ad2. The increased efficiency of wild-type Ad17 could be explained by increased fiber-dependent binding to the epithelia. Therefore, we constructed a chimeric virus, Ad2(17f)beta Gal-2, which is identical to Ad2/beta Gal-2 with the exception of having the fiber protein of Ad17 replace Ad2 fiber. This vector retained the increased binding and efficiency of gene transfer to well-differentiated human airway epithelia. These data suggest that inclusion of Ad17 fiber into adenovirus vectors may improve the outlook for gene delivery to human airway epithelia.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/06/20 alle ore 00:09:02