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Titolo:
Possible interactions between the NS-1 protein and tumor necrosis factor alpha pathways in erythroid cell apoptosis induced by human parvovirus B19
Autore:
Sol, N; Le Junter, J; Vassias, I; Freyssinier, JM; Thomas, A; Prigent, AF; Rudkin, BB; Fichelson, S; Morinet, F;
Indirizzi:
Hop St Louis, F-75475 Paris 10, France Hop St Louis Paris France 10Hop St Louis, F-75475 Paris 10, France Hop Cochin, CNRS, UPR 9051, Lab Rech Hemobiol, F-75674 Paris, France Hop Cochin Paris France F-75674 Lab Rech Hemobiol, F-75674 Paris, France Ecole Normale Super Lyon, CNRS, UMR 49, Lyon, France Ecole Normale Super Lyon Lyon France r Lyon, CNRS, UMR 49, Lyon, France Inst Natl Sci Appl Lyon, INSERM, U352, F-69621 Villeurbanne, France Inst Natl Sci Appl Lyon Villeurbanne France F-69621 Villeurbanne, France
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 10, volume: 73, anno: 1999,
pagine: 8762 - 8770
SICI:
0022-538X(199910)73:10<8762:PIBTNP>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
NF-KAPPA-B; NONSTRUCTURAL PROTEIN; TNF RECEPTOR-1; DEATH-DOMAIN; SPHINGOMYELIN PATHWAY; TRANSCRIPTION FACTOR; INTERFERON-GAMMA; MINUTE VIRUS; GENE-PRODUCT; IN-VITRO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Morinet, F Hop St Louis, 1 Ave Claude Vellefaux, F-75475 Paris 10, France Hop St Louis 1 Ave Claude Vellefaux Paris France 10 10, France
Citazione:
N. Sol et al., "Possible interactions between the NS-1 protein and tumor necrosis factor alpha pathways in erythroid cell apoptosis induced by human parvovirus B19", J VIROLOGY, 73(10), 1999, pp. 8762-8770

Abstract

Human erythroid progenitor cells are the main target cells of the human parvovirus B19 (B19), and B19 infection induces a transient ergthroid aplastic crisis, Several authors have reported that the nonstructural protein 1 (NS-1) encoded by this virus has a cytotoxic effect, but the underlying mechanism of NS-1-induced primary erythroid cell death is still not clear. In human erythroid progenitor cells, we investigated the molecular mechanisms leading to apoptosis after natural infection of these cells by the B19 virus. The cytotoxicity of NS-1 was concomitantly evaluated in transfected erythroid cells. B19 infection and NS-1 expression induced DNA fragmentation characteristic of apoptosis, and the commitment of erythroid cells to undergo apoptosis was combined with their accumulation in the G(2) phase of the cellcycle. Since B19- and NS-1-induced apoptosis was inhibited by caspase 3, 6, and 8 inhibitors, and substantial caspase 3, 6, and 8 activities were induced by NS-1 expression, there may have been interactions between NS-1 and the apoptotic pathways of the death receptors tumor necrosis factor receptor 1 and Fas, Our results suggest that Fas-FasL interaction was not involvedin NS-1- or B19-induced apoptosis in erythroid cells. In contrast, these cells were sensitized to tumor necrosis factor alpha (TNF-alpha)-induced apoptosis. Moreover, the ceramide level was enhanced by B19 infection and NS-1expression. Therefore, our results suggest that there may be a connection between the respective apoptotic pathways activated by TNF-alpha and NS-1 in human erythroid cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 07:45:13