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Titolo:
Simultaneous determination of residual tetracyclines in foods by high-performance liquid chromatography with atmospheric pressure chemical ionizationtandem mass spectrometry
Autore:
Nakazawa, H; Ino, S; Kato, K; Watanabe, T; Ito, Y; Oka, H;
Indirizzi:
Hoshi Univ, Fac Pharmaceut Sci, Dept Analyt Chem, Shinagawa Ku, Tokyo 1428501, Japan Hoshi Univ Tokyo Japan 1428501 Chem, Shinagawa Ku, Tokyo 1428501, Japan Aichi Prefectural Inst Publ Hlth, Kita Ku, Nagoya, Aichi 4628576, Japan Aichi Prefectural Inst Publ Hlth Nagoya Aichi Japan 4628576 628576, Japan
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 732, anno: 1999,
pagine: 55 - 64
SICI:
1387-2273(19990910)732:1<55:SDORTI>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
DISEASED ANIMALS; AICHI PREFECTURE; ANTIBIOTICS; TISSUES; MILK; IMPROVEMENT;
Keywords:
tetracyclines;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Nakazawa, H Hoshi Univ, Fac Pharmaceut Sci, Dept Analyt Chem, Shinagawa Ku, 2-4-41 Ebara, Tokyo 1428501, Japan Hoshi Univ 2-4-41 Ebara Tokyo Japan 1428501 yo 1428501, Japan
Citazione:
H. Nakazawa et al., "Simultaneous determination of residual tetracyclines in foods by high-performance liquid chromatography with atmospheric pressure chemical ionizationtandem mass spectrometry", J CHROMAT B, 732(1), 1999, pp. 55-64

Abstract

We established a method for precisely determining residual tetracycline antibiotics (TCs) in foods by atmospheric pressure chemical ionization liquidchromatography-tandem mass spectrometry (APCI LC-MS-MS) using selected reaction monitoring with an internal standard. By setting the nebulizer probe temperature to 475 degrees C, we were able to use a mobile phase containingoxalic acid without clogging problems at the APCI interface, since oxalic acid decomposes to carbon dioxide and water at high temperature. DMCTC was very effective as an internal standard for determining TCs in various foods. TCs were cleaned up using a Bond Elut ENV cartridge and analysed by APCI LC-MS-MS. The recovery of TCs from various foods including animal tissues, honey, milk, eggs, and fish fortified at levels of 0.05, 0.10, and 0.50 ppmaveraged 60.1-88.9%, with an RSD of 1.2-8.7%. The detection limits were 0.001 ppm for OTC and TC, 0.004 ppm for CTC, and 0.002 ppm for DC, The present method was also successfully used to determine TCs in swine kidney samples that were previously found by microbiological assay. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:24:20