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Titolo:
Determination of 20 underivatized proteinic amino acids by ion-pairing chromatography and pneumatically assisted electrospray mass spectrometry
Autore:
Chaimbault, P; Petritis, K; Elfakir, C; Dreux, M;
Indirizzi:
Univ Orleans, Inst Chim Organ & Analyt, CNRS, UPRES A 6005, Orleans 2, France Univ Orleans Orleans France 2 lyt, CNRS, UPRES A 6005, Orleans 2, France
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY A
fascicolo: 1, volume: 855, anno: 1999,
pagine: 191 - 202
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; ATMOSPHERIC-PRESSURE IONIZATION; AMPEROMETRIC DETECTION; SYSTEM PEAKS; THERMOSPRAY; SEPARATION; PEPTIDES; SPECTRA; AGENTS;
Keywords:
ion-pairing reagents; amino acids; carboxylic acids; perfluorinated;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Elfakir, C Univ Orleans, Inst Chim Organ & Analyt, CNRS, UPRES A 6005, BP 6759, Orleans 2, France Univ Orleans BP 6759 Orleans France 2 6759, Orleans 2, France
Citazione:
P. Chaimbault et al., "Determination of 20 underivatized proteinic amino acids by ion-pairing chromatography and pneumatically assisted electrospray mass spectrometry", J CHROMAT A, 855(1), 1999, pp. 191-202

Abstract

A qualitative determination of 20 underivatized proteinic amino acids by LC-MS is reported. The need for chromatographic separation before mass spectrometry determination is demonstrated based on the study of several amino acid pairs which have some similar characteristics. Two suitable LC-MS systems are proposed for amino acid analysis. A preliminary optimization of these systems has been investigated using evaporative light scattering detection as these two detection modes have the same chromatographic requirements. The amino acid separation was achieved on a Purospher RP-18e or a Supelcosil ABZ(+)Plus column with tridecafluoroheptanoic acid or pentadecafluorooctanoic acid as volatile ion-pairing reagent in an acetonitrile-water mobile phase. In order to elute the most retained amino acids, an elution gradient based on simultaneously increasing the concentration of acetonitrile and decreasing the concentration of the ion-pairing reagent was used. The detection limits of the present work (without specialized optimization) varied from 0.5 to 1 mg l(-1). (C) 1999 Published by Elsevier Science B.V. All rightsreserved.

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Documento generato il 15/07/20 alle ore 14:55:36