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Titolo:
SIMULTANEOUS SOLID-PHASE EXTRACTION, DERIVATIZATION, AND GAS-CHROMATOGRAPHIC MASS-SPECTROMETRIC QUANTIFICATION OF THROMBOXANE AND PROSTACYCLIN METABOLITES, PROSTAGLANDINS, AND ISOPROSTANES IN URINE
Autore:
WUBERT J; REDER E; KASER A; WEBER PC; LORENZ RL;
Indirizzi:
UNIV MUNICH,INST PROPHYLAXIS & EPIDEMIOL CARDIOVASC DIS,PETTENKOFERSTR 9 D-80336 MUNICH GERMANY UNIV MUNICH,INST PROPHYLAXIS & EPIDEMIOL CARDIOVASC DIS D-80336 MUNICH GERMANY
Titolo Testata:
Analytical chemistry
fascicolo: 11, volume: 69, anno: 1997,
pagine: 2143 - 2146
SICI:
0003-2700(1997)69:11<2143:SSEDAG>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT URINE; 2,3-DINOR-THROMBOXANE-B2; 11-DEHYDROTHROMBOXANE-B2; PLASMA; NONCYCLOOXYGENASE; QUANTITATION; PROSTANOIDS; EXCRETION; HUMANS; SERIES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
J. Wubert et al., "SIMULTANEOUS SOLID-PHASE EXTRACTION, DERIVATIZATION, AND GAS-CHROMATOGRAPHIC MASS-SPECTROMETRIC QUANTIFICATION OF THROMBOXANE AND PROSTACYCLIN METABOLITES, PROSTAGLANDINS, AND ISOPROSTANES IN URINE", Analytical chemistry, 69(11), 1997, pp. 2143-2146

Abstract

The current analytical methods for the various prostanoids require a separate and extended sample workup, derivatization, and gas chromatographic/mass spectrometric detection of each compound, Therefore, we developed and validated a rapid method for the common purification, derivatization, and GC/MS determination of 11-dehydrothromboxane B-2, 2,3-dinor-6-keto-PGF(1a), PGF(2a), PGE(2), PGD(2), and isoprostanes in urine. A single reversed-phase solid-phase extraction step and modified reaction conditions yielded excellent sample purification at high recoveries and efficient derivatization for all compounds in one vial. The method allows, for the first time, the simultaneous quantification of these index metabolites of systemic thromboxane and prostacyclin synthesis, renal prostaglandin formation, and nonenzymatic in vivo lipid peroxidation in a single GC/MS run with high sensitivity and precision.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 23:31:23