Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A sodium channel point mutation is associated with resistance to DDT and pyrethroid insecticides in the peach-potato aphid, Myzus persicae (Sulzer) (Hemiptera : Aphididae)
Autore:
Martinez-Torres, D; Foster, SP; Field, LM; Devonshire, AL; Williamson, MS;
Indirizzi:
AFRC, Inst Arable Crops Res, Biol & Ecol Chem Dept, Harpenden AL5 2JQ, Herts, England AFRC Harpenden Herts England AL5 2JQ t, Harpenden AL5 2JQ, Herts, England
Titolo Testata:
INSECT MOLECULAR BIOLOGY
fascicolo: 3, volume: 8, anno: 1999,
pagine: 339 - 346
SICI:
0962-1075(199908)8:3<339:ASCPMI>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
KNOCKDOWN-RESISTANCE; MOLECULAR CHARACTERIZATION; BLATTELLA-GERMANICA; ORGANO-PHOSPHORUS; DNA METHYLATION; GENE-SEQUENCES; KDR; IDENTIFICATION; POPULATIONS; SELECTION;
Keywords:
esterase; insecticide resistance; Myzus persicae; sodium channel;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Williamson, MS AFRC, Inst Arable Crops Res, Biol & Ecol Chem Dept, Harpenden AL5 2JQ, Herts, England AFRC Harpenden Herts England AL5 2JQ 5 2JQ, Herts, England
Citazione:
D. Martinez-Torres et al., "A sodium channel point mutation is associated with resistance to DDT and pyrethroid insecticides in the peach-potato aphid, Myzus persicae (Sulzer) (Hemiptera : Aphididae)", INSEC MOL B, 8(3), 1999, pp. 339-346

Abstract

The voltage-gated sodium channel is the primary target site of DDT and pyrethroid insecticides, and point mutations in the domain II region of the channel protein have been implicated in the knockdown resistant (kdr) phenotype of several insect species. Here, we report that one of these mutations, a leucine-to-phenylalanine replacement in transmembrane segment IIS6, is also found in certain insecticide-resistant clones of the peach-potato aphid,Myzus persicae, The mutation was present in four clones with amplified E4 esterase genes, but was absent from both susceptible clones and those with amplified FE4 genes. The inferred presence of kdr-type resistance in the four E4 clones was subsequently confirmed by bioassays that showed this to bethe primary mechanism of resistance to deltamethrin and DDT, although the esterase-based mechanism also contributes to the overall level of deltamethrin resistance. The kdr mutation on its own conferred 35-fold resistance todeltamethrin and this was enhanced up to 540-fold when it was present in ahigh (E4) esterase background. The esterase (FE4) mechanism was far less effective without the kdr mutation, conferring just 3-4-fold resistance to deltamethrin. These findings, and the linkage disequilibrium of the kdr mutation within clones overproducing the E4 esterase, have important implications for the evolution of resistance in this insect and for the use of pyrethroid sprays in the management of M. persicae populations in the field.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 15:56:59