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Titolo:
In vitro analysis of transformation potential associated with retroviral vector insertions
Autore:
Powell, SK; Kaloss, M; Burimski, I; Weaver, L; Long, ZF; Lyons, R; McGarrity, GJ; Otto, E;
Indirizzi:
Genet Therapy Inc, Gaithersburg, MD 20878 USA Genet Therapy Inc Gaithersburg MD USA 20878 c, Gaithersburg, MD 20878 USA
Titolo Testata:
HUMAN GENE THERAPY
fascicolo: 13, volume: 10, anno: 1999,
pagine: 2123 - 2132
SICI:
1043-0342(19990901)10:13<2123:IVAOTP>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
HISTOCHEMICAL MARKER GENES; MURINE LEUKEMIA-VIRUS; MULTISTAGE CARCINOGENESIS; INTERCELLULAR COMMUNICATION; NONHUMAN-PRIMATES; EGF RECEPTOR; CELLS; MUTAGENESIS; RAS; ONCOGENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Powell, SK Genet Therapy Inc, 938 Clopper Rd, Gaithersburg, MD 20878 USA Genet Therapy Inc 938 Clopper Rd Gaithersburg MD USA 20878 USA
Citazione:
S.K. Powell et al., "In vitro analysis of transformation potential associated with retroviral vector insertions", HUM GENE TH, 10(13), 1999, pp. 2123-2132

Abstract

While replication-defective retroviral vectors provide excellent vehicles for the long-term expression of therapeutic genes, they also harbor the potential to induce undesired genetic changes by random insertions into the host genome. The rate of insertional mutagenesis for retroviral vectors has been determined in several different assay systems; however, the rate at which such events induce cellular transformation has not been directly determined. Such measurements are critical to determining the actual risk of carcinogenesis resulting from retroviral gene therapy. In this study, the ability of a replication-defective retroviral vector, G1nBgSvNa, to induce cellular transformation in the BALB/c-3T3 in vitro transformation assay was assessed. The transformation frequency observed in vector-transduced BALB/c-3T3 cells, which contained one to six copies of integrated provirus, was not significantly different from that of untreated control cells. The finding that G1nBgSvNa was nontransforming in this assay indicates that the rate of transformation induced by retroviral insertions is less than the spontaneous rate of cellular transformation by BALB/c-3T3 cells, or less than 1.1 x 10(-5). These results are the first to define an upper limit for the rate of transformation induced by retroviral vectors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 05:53:12