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Titolo:
Applicability of rabbit microsatellite primers for studies of hybridisation between an introduced and a native hare species
Autore:
Andersson, AC; Thulin, CG; Tegelstrom, H;
Indirizzi:
Uppsala Univ, EBC, Dept Conservat Biol & Genet, S-75007 Uppsala, Sweden Uppsala Univ Uppsala Sweden S-75007 iol & Genet, S-75007 Uppsala, Sweden
Titolo Testata:
HEREDITAS
fascicolo: 3, volume: 130, anno: 1999,
pagine: 309 - 315
SICI:
0018-0661(1999)130:3<309:AORMPF>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
ORYCTOLAGUS-CUNICULUS; ALLELE SIZE; LOCI; CONSTRAINTS; DIFFERENTIATION; CHIMPANZEES; HUMANS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Thulin, CG Uppsala Univ, EBC, Dept Conservat Biol & Genet, Box 7003, S-75007 Uppsala,Sweden Uppsala Univ Box 7003 Uppsala Sweden S-75007 07 Uppsala,Sweden
Citazione:
A.C. Andersson et al., "Applicability of rabbit microsatellite primers for studies of hybridisation between an introduced and a native hare species", HEREDITAS, 130(3), 1999, pp. 309-315

Abstract

Introduced species may hybridise with relatives in the native Fauna or flora and thereby compete for matings and transmit alien DNA. Such interference may contaminate unique genepools, disturb existing ecological balances and may ultimately result in the extinction of the native species. In Sweden,the introduced brown hare (Lepus europaeus Pall.) hybridise with the native mountain hare (L. timidus L.), both relatively common members of the present Swedish fauna. This hybridisation has resulted in the transmission of mitochondrial DNA from the mountain hare to the brown hare, but absence of species differences in karyotype and allozymes have prevented investigationsof the amount of nuclear gene flow. More polymorphic genetic markers are needed to analyse evidence of hybridisation in the nuclear genome. The conservation of microsatellite loci across taxa usually enables PCR amplification of microsatellites in closely related species with the same primers. We have used five microsatellite primer pairs, developed for the European wild rabbit (Oryctolagus cuniculus L.) to amplify microsatellites in the two species of hare in Sweden. The obtained allelic variation was used to construct a generic distance tree based on the amount of shared alleles between ailpairs of individuals (shared-allele index). This method offered sufficientdifferences to arrange all individuals in two groups, one for each species. Identification of individual hybrids based on the number of alleles shared between the species is not possible with these five microsatellite markers.

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Documento generato il 01/10/20 alle ore 00:18:01