Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A putative exchange factor for Rho1 GTPase is required-for initiation of cytokinesis in Drosophila
Autore:
Prokopenko, SN; Brumby, A; OKeefe, L; Prior, L; He, YC; Saint, R; Bellen, HJ;
Indirizzi:
Baylor Coll Med, Program Dev Biol, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 gram Dev Biol, Houston, TX 77030 USA Baylor Coll Med, Howard Hughes Med Inst, Houston, TX 77030 USA Baylor CollMed Houston TX USA 77030 ghes Med Inst, Houston, TX 77030 USA Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA Baylor CollMed Houston TX USA 77030 & Human Genet, Houston, TX 77030 USA Univ Adelaide, Dept Genet, Adelaide, SA 5005, Australia Univ Adelaide Adelaide SA Australia 5005 et, Adelaide, SA 5005, Australia
Titolo Testata:
GENES & DEVELOPMENT
fascicolo: 17, volume: 13, anno: 1999,
pagine: 2301 - 2314
SICI:
0890-9369(19990901)13:17<2301:APEFFR>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLECKSTRIN HOMOLOGY DOMAIN; DBL ONCOGENE PRODUCT; CELL-SHAPE CHANGES; ACTIN-CYTOSKELETON; CONTRACTILE RING; BINDING PROTEIN; 3RD CHROMOSOME; DNA-REPAIR; GENE; MUTATIONS;
Keywords:
cytokinesis; contractile ring; RhoGEF; Rho1 GTPase; pebble;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
68
Recensione:
Indirizzi per estratti:
Indirizzo: Bellen, HJ Baylor Coll Med, Program Dev Biol, Houston, TX 77030 USA BaylorColl Med Houston TX USA 77030 ol, Houston, TX 77030 USA
Citazione:
S.N. Prokopenko et al., "A putative exchange factor for Rho1 GTPase is required-for initiation of cytokinesis in Drosophila", GENE DEV, 13(17), 1999, pp. 2301-2314

Abstract

Cytokinesis ensures the successful completion of the cell cycle and distribution of chromosomes, organelles, and cytoplasm between daughter cells. Itis accomplished by formation and constriction of an actomyosin contractilering that drives the progression of a cleavage furrow. Microinjection experiments and in vitro transfection assays have suggested a requirement for small GTPases of the Rho family in cytokinesis. Yet, the identity of proteins regulating Rho signaling pathways during cytokinesis remains unknown. Here we show that in Drosophila, Pebble (Pbl), a putative exchange factor for Rho GTPases (RhoGEF), is required for the formation of the contractile ringand initiation of cytokinesis. The dynamics of Pbl expression and its distribution during mitosis, as well as structure-function analysis, indicate that it is a key regulatory component of the pathway. pbl interacts genetically with Rho1, but not with Rac1 or Cdc42, and Pbl and Rho1 proteins interact in vivo in yeast. Similar to mutations in pbl, loss of Rho1 or expression of a dominant-negative Rho1 blocks cytokinesis. Our results identify Pbl as a RhoGEF specifically required for cytokinesis and linked through Rho1 activity to the reorganization of the actin cytoskeleton at the cleavage furrow.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 10:24:59