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Titolo:
Phylogenetic analysis of algicidal bacteria (Family Flavobacteriaceae) andselective detection by PCR using a specific primer set
Autore:
Kondo, R; Imai, I; Fukami, K; Minami, A; Hiroishi, S;
Indirizzi:
Fukui Prefectural Univ, Dept Marine Biosci, Obama, Fukui 9170003, Japan Fukui Prefectural Univ Obama Fukui Japan 9170003 ma, Fukui 9170003, Japan Kyoto Univ, Grad Sch Agr, Div Appl Biosci, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 gr, Div Appl Biosci, Kyoto 6068502, Japan Kochi Univ, Fac Agr, Dept Aquaculture, Nanko Ku, Kochi 7838502, Japan Kochi Univ Kochi Japan 7838502 uaculture, Nanko Ku, Kochi 7838502, Japan
Titolo Testata:
FISHERIES SCIENCE
fascicolo: 3, volume: 65, anno: 1999,
pagine: 432 - 435
SICI:
0919-9268(199904)65:3<432:PAOAB(>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLIDING BACTERIUM; ALIGNMENT;
Keywords:
algicidal bacteria; Cytophaga; Flavobacterium; 16S rDNA; PCR; Chattonella antiqua; Heterocapsa circularisquama;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Kondo, R Fukui Prefectural Univ, Dept Marine Biosci, Obama, Fukui 9170003,Japan Fukui Prefectural Univ Obama Fukui Japan 9170003 9170003, Japan
Citazione:
R. Kondo et al., "Phylogenetic analysis of algicidal bacteria (Family Flavobacteriaceae) andselective detection by PCR using a specific primer set", FISHERIES S, 65(3), 1999, pp. 432-435

Abstract

Sequencing the 16S rDNA and phylogenetic analysis were performed on algicidal bacteria belonging to the family Flavobacteriaceae, Chattonella-killingCytophaga sp. strain J18/M01, Heterocapsa-killing Cytophaga sp. strains AA8-2 and AA8-3, and Gymnodinium growth-inhibiting Flavobacterium sp. strain 5N-3. These algicidal strains were clustered phylogenetically within the marine Cytophaga/Flavobacter/Bacteroides (CFB) group. Flavobacterium sp. 5N-3was phylogenetically clustered with Psychroserpens burtonensis but not with the algicidal gliding bacteria. The sequence similarity between strain 5N-3 and P. burtonensis was only 92%. The strains J18/M01 and AA8-2 (AA8-3) exhibited the high level of sequence similarity of 98.5%. Despite the differences of host algae and of locality and year of isolation, these algicidal bacteria were phylogenetically located on the same terminal branch. The PCR amplification of the 16S rDNA of the CFB strains using a single set of J18/M01-specific primers resulted in specific detection of the J18/M01, indicating that the J18/M01-specific sequence in the 16S rDNA is useful for selective detection of the strain J18/M01.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 07:34:54