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Titolo:
Problems in predicting cell damage from bubble bursting
Autore:
Dey, D; Emery, AN;
Indirizzi:
Univ Birmingham, Sch Chem Engn, Birmingham B15 2TT, W Midlands, England Univ Birmingham Birmingham W Midlands England B15 2TT W Midlands, England
Titolo Testata:
BIOTECHNOLOGY AND BIOENGINEERING
fascicolo: 2, volume: 65, anno: 1999,
pagine: 240 - 245
SICI:
0006-3592(19991020)65:2<240:PIPCDF>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTECTING ADDITIVES; SPARGED BIOREACTORS; ANIMAL-CELLS; GAS-BUBBLES; SURFACTANT; INJURY;
Keywords:
cell damage; bubble burst; surface properties; surfactants;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Emery, AN Univ Birmingham, Sch Chem Engn, Birmingham B15 2TT, W Midlands, England Univ Birmingham Birmingham W Midlands England B15 2TT , England
Citazione:
D. Dey e A.N. Emery, "Problems in predicting cell damage from bubble bursting", BIOTECH BIO, 65(2), 1999, pp. 240-245

Abstract

The question is addressed as to whether observed parameter(s) characterizing single bubble burst (bubble jet height and speed) can be used to predictcell damage in sparged animal cell cultures. Bubble burst profiles are examined in the presence of realistic concentrations of fetal calf serum (FCS)or Pluronic F-68 using a high-speed video technique. The damage to TBC3 hybridoma cells from bubble sparging, characterized as a first-order decline,is reduced by even very small concentrations of both FCS and Pluronic F-68, but neither single bubble burst parameters nor surface properties give useable correlations with death rate constants. (C) 1999 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 21:23:53