Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
In vitro analysis of the glucose-transport system in GLUT4-null skeletal muscle
Autore:
Ryder, JW; Kawano, Y; Chibalin, AV; Rincon, J; Tsao, TS; Stenbit, AE; Combatsiaris, T; Yang, J; Holman, GD; Charron, MJ; Zierath, JR;
Indirizzi:
Karolinska Hosp, Dept Clin Physiol, S-17176 Stockholm, Sweden Karolinska Hosp Stockholm Sweden S-17176 siol, S-17176 Stockholm, Sweden Albert Einstein Coll Med, Dept Biochem, Bronx, NY 10461 USA Albert Einstein Coll Med Bronx NY USA 10461 Biochem, Bronx, NY 10461 USA Univ Bath, Dept Biochem, Bath BA2 7AY, Avon, England Univ Bath Bath Avon England BA2 7AY Biochem, Bath BA2 7AY, Avon, England
Titolo Testata:
BIOCHEMICAL JOURNAL
, volume: 342, anno: 1999,
parte:, 2
pagine: 321 - 328
SICI:
0264-6021(19990901)342:<321:IVAOTG>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
INSULIN-RESPONSIVE TISSUES; RAT ADIPOSE-CELLS; 3T3-L1 CELLS; SMALL-INTESTINE; INTRACELLULAR POOL; LOCALIZATION; GLUT5; STIMULATION; METABOLISM; IDENTIFICATION;
Keywords:
GLUT1; GLUT3; GLUT4 knock-out; GLUT5; insulin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Zierath, JR Karolinska Hosp, Dept Clin Physiol, S-17176 Stockholm, Sweden Karolinska Hosp Stockholm Sweden S-17176 6 Stockholm, Sweden
Citazione:
J.W. Ryder et al., "In vitro analysis of the glucose-transport system in GLUT4-null skeletal muscle", BIOCHEM J, 342, 1999, pp. 321-328

Abstract

We have characterized the glucose-transport system in soleus muscle from female GLUT4-null mice to determine whether GLUT1, 3 or 5 account for insulin-stimulated glucose-transport activity. Insulin increased 2-deoxyglucose uptake 2.8- and 2.1-fold in soleus muscle from wild-type and GLUT4-null mice, respectively. Cytochalasin B, an inhibitor of GLUT1- and GLUT4-mediated glucose transport, inhibited insulin-stimulated 2-deoxyglucose uptake by > 95% in wild-type and GLUT4-null soleus muscle. Addition of 35 mM fructose tothe incubation media was without effect on insulin-stimulated 3-O-methyl-glucose transport activity in soleus muscle from either genotype, whereas 35mM glucose inhibited insulin-stimulated (20 nM) 3-O-methylglucose transport by 65% in wild-type and 99% in GLUT4-null mice. We utilized the 2-N-4-1-(1-azi-2,2,2-trifluoroethyl)benzoyl-1,3-bis(D-mannose-4-yloxy)-2-propylamine(ATB-BMPA) exofacial photolabel to determine if increased cell-surface GLUT1 or GLUT4 content accounted for insulin-stimulated glucose transport in GLUT4-null muscle. In wild-type soleus muscle, cell-surface GLUT4 content was increased by 2.8-fold under insulin-stimulated conditions and this increase corresponded to the increase in 2-deoxyglucose uptake. No detectable cell-surface GLUT4 was observed in soleus muscle from female GLUT4-null mice under either basal or insulin-stimulated conditions. Basal cell-surface GLUT1 content was similar between wild-type and GLUT4-null mice, with no further increase noted in either genotype with insulin exposure. Neither GLUT3 nor GLUTS appeared to account for insulin-stimulated glucose-transport activity in wild-type or GLUT4-null muscle. In conclusion, insulin-stimulated glucose-transport activity in female GLUT4-null soleus muscle is mediated by afacilitative transport process that is glucose- and cytochalasin B-inhibitable, but which is not labelled strongly by ATB-BMPA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 12:08:26