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Titolo:
Vascular channel formation by human melanoma cells in vivo and in vitro: Vasculogenic mimicry
Autore:
Maniotis, AJ; Folberg, R; Hess, A; Seftor, EA; Gardner, LMG; Peer, J; Trent, JM; Meltzer, PS; Hendrix, MJC;
Indirizzi:
Univ Iowa, Ctr Canc, Dept Anat, Iowa City, IA USA Univ Iowa Iowa City IA USA Iowa, Ctr Canc, Dept Anat, Iowa City, IA USA Univ Iowa, Ctr Canc, Dept Cell Biol, Iowa City, IA USA Univ Iowa Iowa City IA USA , Ctr Canc, Dept Cell Biol, Iowa City, IA USA Univ Iowa, Coll Med, Dept Ophthalmol & Visual Sci, Iowa City, IA USA Univ Iowa Iowa City IA USA pt Ophthalmol & Visual Sci, Iowa City, IA USA Univ Iowa, Coll Med, Dept Pathol, Iowa City, IA USA Univ Iowa Iowa City IA USA owa, Coll Med, Dept Pathol, Iowa City, IA USA Hadassah Univ Hosp, Dept Ophthalmol, IL-91120 Jerusalem, Israel Hadassah Univ Hosp Jerusalem Israel IL-91120 IL-91120 Jerusalem, Israel Natl Human Genome Res Inst, Canc Genet Branch, NIH, Bethesda, MD USA Natl Human Genome Res Inst Bethesda MD USA Branch, NIH, Bethesda, MD USA
Titolo Testata:
AMERICAN JOURNAL OF PATHOLOGY
fascicolo: 3, volume: 155, anno: 1999,
pagine: 739 - 752
SICI:
0002-9440(199909)155:3<739:VCFBHM>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
UVEAL MALIGNANT-MELANOMA; CILIARY BODY MELANOMAS; 10 LARGEST NUCLEOLI; MICROCIRCULATION ARCHITECTURE; INTERMEDIATE FILAMENTS; CHOROIDAL MELANOMAS; HUMAN CYTOTROPHOBLASTS; ENDOVASCULAR INVASION; LASER OPHTHALMOSCOPY; EXTRACELLULAR-MATRIX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Hendrix, MJC Univ Iowa, Coll Med, Dept Anat & Cell Biol, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 ol, Iowa City, IA 52242 USA
Citazione:
A.J. Maniotis et al., "Vascular channel formation by human melanoma cells in vivo and in vitro: Vasculogenic mimicry", AM J PATH, 155(3), 1999, pp. 739-752

Abstract

Tissue sections from aggressive human intraocular (uveal) and metastatic cutaneous melanomas generally lack evidence of significant necrosis and contain patterned networks of interconnected loops of extracellular matrix. Thematrix that forms these loops or networks may be solid or hollow. Red blood cells have been detected within the hollow channel components of this patterned matrix histologically, and these vascular channel networks have beendetected in human tumors angiographically, Endothelial cells were not identified within these matrix-embedded channels by light microscopy, by transmission electron microscopy, or by using an immunohistochemical panel of endothelial cell markers (Factor VIII-related antigen, Ulex, CD31, CD34, and KDR[Flk-1]). Highly invasive primary and metastatic human melanoma cells formed patterned solid and hollow matrix channels (seen in tissue sections of aggressive primary and metastatic human melanomas) in three-dimensional cultures containing Matrigel or dilute Type I collagen, without endothelial cells or fibroblasts. These tumor cell-generated patterned channels conducteddye, highlighting looping patterns visualized angiographically in human tumors. Neither normal melanocytes nor poorly invasive melanoma cells generated these patterned channels in vitro under identical culture conditions, even after the addition of conditioned medium from metastatic pattern-formingmelanoma cells, soluble growth factors, or regimes of hypoxia, Highly invasive and metastatic human melanoma cells, but clot poorly invasive melanomacells, contracted and remodeled floating hydrated gels, providing a biomechanical explanation for the generation of microvessels in vitro. cDNA microarray analysis of highly invasive versus poorly invasive melanoma tumor cells confirmed a genetic reversion to a pluripotent embryonic-like genotype in the highly aggressive melanoma cells. These observations strongly suggestthat aggressive melanoma cells may generate vascular channels that facilitate tumor perfusion independent of tumor angiogenesis.

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Documento generato il 02/10/20 alle ore 01:30:30