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Titolo:
ROX, A NOVEL BHLHZIP PROTEIN EXPRESSED IN QUIESCENT CELLS THAT HETERODIMERIZES WITH MAX, BINDS A NONCANONICAL E-BOX AND ACTS AS A TRANSCRIPTIONAL REPRESSOR
Autore:
MERONI G; REYMOND A; ALCALAY M; BORSANI G; TANIGAMI A; TONLORENZI R; LONIGRO C; MESSALI S; ZOLLO M; LEDBETTER DH; BRENT R; BALLABIO A; CARROZZO R;
Indirizzi:
TELETHON INST GENET & MED,SAN RAFFAELE BIOMED SCI PK MILAN ITALY TELETHON INST GENET & MED MILAN ITALY HARVARD UNIV,SCH MED,DEPT GENET BOSTON MA 00000 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT MOL BIOL BOSTON MA 00000 IST EUROPEO ONCOL MILAN ITALY NATL CANC CTR,RES INST TOKYO 104 JAPAN UNIV CHICAGO,CTR GENET MED CHICAGO IL 60637 HOSP SAN RAFFAELE,SERV GENET MED I-20132 MILAN ITALY
Titolo Testata:
EMBO journal
fascicolo: 10, volume: 16, anno: 1997,
pagine: 2892 - 2906
SICI:
0261-4189(1997)16:10<2892:RANBPE>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN C-MYC; LOOP-HELIX PROTEIN; TUMOR-SUPPRESSOR GENE; CASEIN KINASE-II; DNA-BINDING; ACTIVATES TRANSCRIPTION; N-MYC; NEOPLASTIC TRANSFORMATION; TUMORIGENIC CONVERSION; NUCLEAR-LOCALIZATION;
Keywords:
BHLHZIP PROTEINS; MAX-INTERACTING PROTEINS; MYC; SIN3; TRANSCRIPTIONAL REPRESSOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
93
Recensione:
Indirizzi per estratti:
Citazione:
G. Meroni et al., "ROX, A NOVEL BHLHZIP PROTEIN EXPRESSED IN QUIESCENT CELLS THAT HETERODIMERIZES WITH MAX, BINDS A NONCANONICAL E-BOX AND ACTS AS A TRANSCRIPTIONAL REPRESSOR", EMBO journal, 16(10), 1997, pp. 2892-2906

Abstract

Proteins of the Myc and Mad family are involved in transcriptional regulation and mediate cell differentiation and proliferation, These molecules share a basic-helix-loop-helix leucine zipper domain (bHLHZip) and bind DNA at the E box (CANNTG) consensus by forming heterodimers with Max, We report the isolation, characterization and mapping of a human gene and its mouse homolog encoding a new member of this family ofproteins, named Pox, Through interaction mating and immunoprecipitation techniques, we demonstrate that Rox heterodimerizes with Max and weakly homodimerizes, Interestingly, bandshift assays demonstrate that the Fox-Max heterodimer shows a novel DNA binding specificity, having ahigher affinity for the CACGCG site compared with the canonical E boxCACGTG site, Transcriptional studies indicate that Fox represses transcription in both human HEK293 cells and yeast. We demonstrate that repression in yeast is through interaction between the N-terminus of theprotein and the Sin3 co-repressor, as previously shown for the other Mad family members, ROX is highly expressed in quiescent fibroblasts and expression markedly decreases when cells enter the cell cycle. Moreover, ROX expression appears to be induced in U937 myeloid leukemia cells stimulated to differentiate with 12-O-tetradecanoylphorbol-13-acetate, The identification of a novel Max-interacting protein adds an important piece to the puzzle of Myc/Max/Mad coordinated action and function in normal and pathological situations. Furthermore, mapping of thehuman gene to chromosome 17p13.3 in a region that frequently undergoes loss of heterozygosity in a number of malignancies, together with the biochemical and expression features, suggest involvement of ROX in human neoplasia.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:39:44