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Titolo:
The avian egg as a test system for endocrine disrupters: effects of diethylstilbestrol and ethynylestradiol on sex organ development
Autore:
Berg, C; Halldin, K; Fridolfsson, AK; Brandt, I; Brunstrom, B;
Indirizzi:
Dept Environm Toxicol, S-75236 Uppsala, Sweden Dept Environm Toxicol Uppsala Sweden S-75236 ol, S-75236 Uppsala, Sweden Swedish Univ Agr Sci, Uppsala Biomed Ctr, Dept Anim Breeding & Genet, S-75124 Uppsala, Sweden Swedish Univ Agr Sci Uppsala Sweden S-75124 net, S-75124 Uppsala, Sweden
Titolo Testata:
SCIENCE OF THE TOTAL ENVIRONMENT
fascicolo: 1-3, volume: 233, anno: 1999,
pagine: 57 - 66
SICI:
0048-9697(19990815)233:1-3<57:TAEAAT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESTROGEN-RECEPTOR; CHICKEN EMBRYOS; AROMATASE; EXPRESSION; 3,3',4,4'-TETRACHLOROBIPHENYL; HYDROXYLASE; CHEMICALS; GENES;
Keywords:
endocrine disruption; environmental estrogens; reproductive organ morphology; ovotestis; test system; biomarkers; Japanese quail; ethynylestradiol; diethylstilbestrol;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Berg, C Dept Environm Toxicol, Norbyvagen 18A, S-75236 Uppsala, Sweden Dept Environm Toxicol Norbyvagen 18A Uppsala Sweden S-75236 weden
Citazione:
C. Berg et al., "The avian egg as a test system for endocrine disrupters: effects of diethylstilbestrol and ethynylestradiol on sex organ development", SCI TOTAL E, 233(1-3), 1999, pp. 57-66

Abstract

Many environmental contaminants are known or suspected to interfere with hormonal function in animals. In vivo test methods to detect and characterize chemicals that disrupt the endocrine system are therefore urgently needed. In this study, we assessed the usefulness of abnormalities of the reproductive organs as test endpoints for estrogenic activity of xenobiotics in Japanese quail embryos. Two synthetic estrogens, diethylstilbestrol (DES) andethynylestradiol (EE2), were injected into the yolks of embryonated eggs. At a dose as low as 2 ng EE2/g egg, all male embryos became feminized, containing ovary-like tissue in the left testis. The extent of feminization of the testes was determined by measuring the relative area of the ovary-like component. Persistent Mullerian ducts (oviducts) in male embryos, and malformations of the Mullerian ducts in females occurred at 2 ng EE2/g egg and higher doses. DES was approximately one-third to one-tenth as potent as EE2. The morphological changes studied were dose-dependent, indicating that they are useful as test endpoints for estrogenic activity. Feminization of theleft testis in males proved to be the most sensitive endpoint. We propose the quail egg as a simple in vivo test system for estrogenic compounds. (C)1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 21:34:03