Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The development of an automated in situ assay for the detection of human cytomegalovirus in peripheral blood leukocytes
Autore:
Baunoch, DA; Lobell, A; Kane, D; Fredericks, J; Thompson, KD;
Indirizzi:
Univ Chicago, Med Ctr, Dept Pathol, Chicago, IL 60637 USA Univ Chicago Chicago IL USA 60637 Ctr, Dept Pathol, Chicago, IL 60637 USA Ventana Med Syst, Tucson, AZ USA Ventana Med Syst Tucson AZ USAVentana Med Syst, Tucson, AZ USA
Titolo Testata:
JOURNAL OF VIROLOGICAL METHODS
fascicolo: 1-2, volume: 81, anno: 1999,
pagine: 31 - 37
SICI:
0166-0934(199908)81:1-2<31:TDOAAI>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACQUIRED IMMUNODEFICIENCY SYNDROME; BONE-MARROW TRANSPLANTATION; VIRUS INFECTION; INSITU HYBRIDIZATION; DISEASE; LOCALIZATION; ANTIGENEMIA; GANCICLOVIR; RECIPIENTS; CULTURES;
Keywords:
human cytomegalovirus; in situ hybridization; immediate early mRNA; leukocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Thompson, KD Univ Chicago, Med Ctr, Dept Pathol, MC 0001,5841 S Maryland Ave, Chicago, IL 60637 USA Univ Chicago MC 0001,5841 S Maryland Ave Chicago IL USA 60637
Citazione:
D.A. Baunoch et al., "The development of an automated in situ assay for the detection of human cytomegalovirus in peripheral blood leukocytes", J VIROL MET, 81(1-2), 1999, pp. 31-37

Abstract

Human cytomegalovirus (HCMV) infections are common in immunosuppressed patients, especially transplant recipients and patients with AIDS. The utilityof an automated in situ hybridization (ISH) assay for the rapid detection of HCMV immediate early mRNA was evaluated using cytospin (Shandon Lipshaw,Inc., Pittsburgh, PA) prepared leukocytes from peripheral blood samples. In this study, the detection of HCMV immediate early protein by immunofluorescent antibody staining of the standard shell vial assay was compared to the detection of HCMV immediate early mRNA in peripheral blood leukocytes using the automated ISH system. Of 135 specimens tested, eight specimens were positive using HCMV ISH compared to seven positive specimens using shell vial assay. Overall, HCMV ISH demonstrated 100% sensitivity and 99% specificity. Since the HCMV ISH assay requires minimal labor, and can be completed in less than 5 h, this method should be evaluated as a potential replacementfor shell vial assay for the diagnosis of HCMV infection. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 19:06:29