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Titolo:
Age-related effects of St Thomas' Hospital cardioplegic solution on isolated cardiomyocyte cell volume
Autore:
Danetz, JS; Clemo, HF; Davies, RD; Embrey, RP; Damiano, RJ; Baumgarten, CM;
Indirizzi:
Virginia Commonwealth Univ, Med Coll Virginia, Dept Physiol, Richmond, VA 23298 USA Virginia Commonwealth Univ Richmond VA USA 23298 , Richmond, VA 23298 USA Virginia Commonwealth Univ, Med Coll Virginia, Dept Surg, Div CardiothoracSurg, Richmond, VA 23298 USA Virginia Commonwealth Univ Richmond VA USA 23298 , Richmond, VA 23298 USA Virginia Commonwealth Univ, Med Coll Virginia, Dept Internal Med, Div Cardiol, Richmond, VA 23298 USA Virginia Commonwealth Univ Richmond VA USA 23298 , Richmond, VA 23298 USA Penn State Univ, Milton S Hershey Med Ctr, Geisinger Hlth Syst, Sect Cardiothorac Surg, Hershey, PA 17033 USA Penn State Univ Hershey PA USA 17033 iothorac Surg, Hershey, PA 17033 USA
Titolo Testata:
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY
fascicolo: 3, volume: 118, anno: 1999,
pagine: 467 - 476
SICI:
0022-5223(199909)118:3<467:AEOSTH>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEVELOPMENTAL-CHANGES; CRYSTALLOID CARDIOPLEGIA; VENTRICULAR MYOCYTES; ISCHEMIC ARREST; RABBIT ATRIAL; HEART; EDEMA; PROTECTION; PREVENTION; MYOCARDIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Baumgarten, CM Virginia Commonwealth Univ, Med Coll Virginia, Dept Physiol, Richmond, VA 23298 USA Virginia Commonwealth Univ Richmond VA USA 23298 23298 USA
Citazione:
J.S. Danetz et al., "Age-related effects of St Thomas' Hospital cardioplegic solution on isolated cardiomyocyte cell volume", J THOR SURG, 118(3), 1999, pp. 467-476

Abstract

Objectives: We tested the hypothesis that neonatal cells are more sensitive to cardioplegia-induced cell swelling than more mature cells and spontaneous swelling in the absence of ischemia can be prevented by cardioplegia with a physiologic KCl product. Methods: Cell volumes of isolated ventricularmyocytes from neonatal (3-5 days), intermediate (10-13 days), and adult (>6 weeks) rabbits were measured by digital video microscopy. After equilibration in 37 degrees C physiologic solution, cells were suprafused with 37 degrees C or 9 degrees C St Thomas' Hospital solution (standard or low Cl-) or 9 degrees C physiologic solution followed by reperfusion with 37 degrees C physiologic solution. Results: Neonatal cells swelled 16.2% +/- 1.8% (P <.01) in 37 degrees C St Thomas' Hospital solution and recovered during reperfusion, whereas more mature cells maintained constant volume, In contrast, 9 degrees C St Thomas' Hospital solution caused significant age-dependentswelling (neonatal, 16.8% +/- 1.5%; intermediate, 8.6% +/- 2.1%; adult, 5.6% +/- 1.1%). In contrast to more mature cells, neonatal cells remained significantly edematous throughout reperfusion (8.1% +/- 1.5%). Swelling was not due to hypothermia because 9 degrees C physiologic solution did not affect volume, Lowering the KCl product of St Thomas' Hospital solution by partially replacing Cl- with an impermeant anion prevented cellular edema in all groups, Conclusion: In the absence of ischemia, neonatal cells were more sensitive to cardioplegia-induced cellular edema than more mature cells, and edema observed in all groups was avoided by decreasing the KCl product ofSt Thomas' Hospital solution to the physiologic range. Differences in cellvolume regulation may explain the sensitivity of neonatal hearts to hyperkalemic cardioplegic arrest and suggest novel approaches to improving myocardial protection.

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Documento generato il 20/09/20 alle ore 04:43:45