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Titolo:
Simultaneous determination of artemether and its major metabolite dihydroartemisinin in plasma by gas chromatography-mass spectrometry-selected ion monitoring
Autore:
Mohamed, SS; Khalid, SA; Ward, SA; Wan, TSM; Tang, HPO; Zheng, M; Haynes, RK; Edwards, G;
Indirizzi:
Univ Khartoum, Fac Pharm, Dept Pharmaceut, Khartoum, Sudan Univ Khartoum Khartoum Sudan ac Pharm, Dept Pharmaceut, Khartoum, Sudan Univ Khartoum, Fac Pharm, Dept Pharmacognosy, Khartoum, Sudan Univ Khartoum Khartoum Sudan Pharm, Dept Pharmacognosy, Khartoum, Sudan Univ Liverpool, Dept Pharmacol & Therapeut, Liverpool L69 3GE, Merseyside,England Univ Liverpool Liverpool Merseyside England L69 3GE E, Merseyside,England Univ Liverpool, Liverpool Sch Trop Med, Liverpool L3 5QA, Merseyside, England Univ Liverpool Liverpool Merseyside England L3 5QA A, Merseyside, England Hong Kong Univ Sci & Technol, Dept Chem, Hong Kong, Peoples R China Hong Kong Univ Sci & Technol Hong Kong Peoples R China Peoples R China
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 731, anno: 1999,
pagine: 251 - 260
SICI:
1387-2273(19990820)731:2<251:SDOAAI>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; REDUCTIVE ELECTROCHEMICAL DETECTION; ULTRAVIOLET DETECTION; ANTIMALARIAL ARTEETHER; ARTEMISIA-ANNUA; ARTESUNIC ACID; BLOOD-PLASMA; PHARMACOKINETICS; DERIVATIZATION; DERIVATIVES;
Keywords:
artemether; dihydroartemisinin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Mohamed, SS Univ Khartoum, Fac Pharm, Dept Pharmaceut, POB 1996, Khartoum,Sudan Univ Khartoum POB 1996 Khartoum Sudan 1996, Khartoum, Sudan
Citazione:
S.S. Mohamed et al., "Simultaneous determination of artemether and its major metabolite dihydroartemisinin in plasma by gas chromatography-mass spectrometry-selected ion monitoring", J CHROMAT B, 731(2), 1999, pp. 251-260

Abstract

A sensitive, selective, and reproducible GC-MS-SIM method was developed for determination of artemether (ARM) and dihydroartemisinin (DHA) in plasma using artemisinin (ART) as internal standard. Solid phase extraction was performed using C-18 Bond Elut cartridges. The analysis was carried out usinga HP-5MS 5% phenylmethylsiloxane capillary column. The recoveries of ARM, DHA and ART were 94.9+/-1.6%, 92.2+/-4.1% and 81.3+/-1.2%, respectively. The limit of quantification in plasma was 5 ng/ml (C.V.less than or equal to 17.4% for ARM and 15.2% for DHA). Calibration curves were linear with R(2)greater than or equal to 0.988. Within day coefficients of variation were 3-10.4% for ARM and7.7-14.5% for DHA. Between day coefficients of variations were 6.5-15.4% and 7.6-14.1% for ARM and:DHA. The method is currently beingused for pharmacokinetic studies. Preliminary data on pharmacokinetics showed C-max of 245.2 and 35.6 nglml reached at 2 and 3 h and AUC(0-8h), of 2463.6 and 111.8 ngh/ml for ARM and DHA, respectively. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 19:29:00