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Titolo:
Fishing for a drug: solid-phase microextraction for the assay of clozapinein human plasma
Autore:
Ulrich, S; Kruggel, S; Weigmann, H; Hiemke, C;
Indirizzi:
Otto Von Guericke Univ, Univ Hosp, Inst Clin Pharmacol, D-39120 Magdeburg,Germany Otto Von Guericke Univ Magdeburg Germany D-39120 39120 Magdeburg,Germany Univ Mainz, Univ Hosp, Psychiat Clin, D-55101 Mainz, Germany Univ Mainz Mainz Germany D-55101 , Psychiat Clin, D-55101 Mainz, Germany
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 731, anno: 1999,
pagine: 231 - 240
SICI:
1387-2273(19990820)731:2<231:FFADSM>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAPILLARY GAS-CHROMATOGRAPHY; PHOSPHORUS SELECTIVE DETECTION; LIQUID-CHROMATOGRAPHY; MASS-SPECTROMETRY; MAJOR METABOLITES; HUMAN BLOOD; HUMAN-SERUM; URINE; ACID;
Keywords:
solid-phase microextraction; clozapine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Ulrich, S Otto Von Guericke Univ, Univ Hosp, Inst Clin Pharmacol, Leipziger Str 44, D-39120 Magdeburg, Germany Otto Von Guericke Univ Leipziger Str 44 Magdeburg Germany D-39120
Citazione:
S. Ulrich et al., "Fishing for a drug: solid-phase microextraction for the assay of clozapinein human plasma", J CHROMAT B, 731(2), 1999, pp. 231-240

Abstract

Solid-phase microextraction (SPME) was investigated as a sample preparation method for assaying the neuroleptic drug clozapine in human plasma. A mixture of human plasma, water; loxapine (as internal standard) and aqueous NaOH was extracted with a 100-mu m polydimethylsiloxane (PDMS) fiber (Supelco). Desorption of the fiber was performed in the injection port of a gas chromatograph at 260 degrees C (HP 5890; 30 mx0.53 mm I.D., 1 mu m film capillary; nitrogen-phosphorous selective detection). Fibers were used repeatedlyin up to about 75 analyses. The recovery was found to be 3% for clozapine from plasma after 30 min of extraction. However, in spite of the low recovery, the analyte was well separated and the calibration was linear between 100 and 1000 ng/ml. The within-day and between-day precision was consistently about 8 to 15% at concentrations of 200 ng/ml to 1000 ng/ml. No interfering drug was found. The limit of detection was 30 ng/ml. The sample volume was 250 mu l. The influence of the concentration of proteins, triglycerides and salt, i.e., changes in the matrix on the peak areas and peak-area ratios was studied. The method is not impaired by physiological changes in the composition of the matrix. Good agreement was found with a liquid-liquid extraction-gas-liquid chromatography (LLE-GLC) standard method and an on-line column-switching high-performance liquid chromatography (HPLC) method for patients' samples and spiked samples, respectively. It is concluded that themethod can be used in the therapeutic drug monitoring of clozapine becausethe therapeutic window of clozapine is from 350 to 600 ng/ml. (C) 1999 Elsevier Science BN. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 10:21:07