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Titolo:
The functional diversity of epidermal keratins revealed by the partial rescue of the Keratin 14 null phenotype by Keratin 16
Autore:
Paladini, RD; Coulombe, PA;
Indirizzi:
Johns Hopkins Univ, Sch Med, Dept Biol Chem, Baltimore, MD 21205 USA JohnsHopkins Univ Baltimore MD USA 21205 l Chem, Baltimore, MD 21205 USA Johns Hopkins Univ, Sch Med, Dept Dermatol, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 rmatol, Baltimore, MD 21205 USA
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 5, volume: 146, anno: 1999,
pagine: 1185 - 1201
SICI:
0021-9525(19990906)146:5<1185:TFDOEK>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSGENIC MOUSE SKIN; EPIDERMOLYSIS-BULLOSA SIMPLEX; STRATIFIED SQUAMOUS EPITHELIA; WHITE SPONGE NEVUS; PACHYONYCHIA-CONGENITA; BASAL CELLS; INTERMEDIATE FILAMENTS; MOLECULAR MARKERS; WOUND REPAIR; STEM-CELLS;
Keywords:
epidermis; hair; keratin; transgenic mice; gene replacement;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
66
Recensione:
Indirizzi per estratti:
Indirizzo: Coulombe, PA Johns Hopkins Univ, Sch Med, Dept Biol Chem, 725 N Wolfe St, Baltimore, MD21205 USA Johns Hopkins Univ 725 N Wolfe St Baltimore MD USA 21205 USA
Citazione:
R.D. Paladini e P.A. Coulombe, "The functional diversity of epidermal keratins revealed by the partial rescue of the Keratin 14 null phenotype by Keratin 16", J CELL BIOL, 146(5), 1999, pp. 1185-1201

Abstract

The type I epidermal keratins K14 and K16 are remarkably similar at the primary sequence level. While a structural function has been clearly defined for K14, we have proposed that a function of K16 may be to play a role in the process of keratinocyte activation that occurs after acute injury to stratified epithelia. To compare directly the functions of the two keratins wehave targeted the expression of the human K16 cDNA to the progenitor basallayer of the epidermis of K14 null mice. Mice null for K14 blister extensively and die similar to 2 d after birth (Lloyd, C., Q.C. Yu, J. Cheng, K. Turksen, L. Degenstein, E. Hutton, and E. Fuchs 1995. J. Cell Biol. 129:1329-1344). The skin of mice expressing K16 in the absence of K14 developed normally without evidence of blistering, However, as the mice aged they featured extensive alopecia, chronic epidermal ulcers in areas of frequent physical contact, and alterations in other stratified epithelia. Mice expressing a control K16-C14 cDNA also rescue the blistering phenotype of the K14 nullmice with only a small percentage exhibiting minor alopecia. While K16 is capable of rescuing the blistering, phenotypic complementation in the resulting skin is incomplete due to the multiple age dependent anomalies. Despite their high sequence similarity, K16 and K14 are not functionally equivalent in the epidermis and other stratified epithelia and it is primarily the carboxy-terminal similar to 105 amino acids of K16 that define these differences.

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Documento generato il 29/11/20 alle ore 16:20:03