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Titolo:
Chemotactic activity of culture supernatants of free and encapsulated pancreatic rat islets towards peritoneal macrophages
Autore:
Karsten, V; Lencioni, C; Tritschler, S; Marchetti, P; Belcourt, A; Navalesi, R; Alexandre, E; Poindron, P; Pinget, M; Kessler, L;
Indirizzi:
Univ Strasbourg 1, Lab Endocrinol Expt, UPRES ULP 2106, CeeD, F-67070 Strasbourg, France Univ Strasbourg 1 Strasbourg France F-67070 , F-67070 Strasbourg, France Univ Pisa, Ist Clin Med 2, I-56100 Pisa, Italy Univ Pisa Pisa Italy I-56100 v Pisa, Ist Clin Med 2, I-56100 Pisa, Italy Fondat Transplantat, Strasbourg, France Fondat Transplantat Strasbourg France Transplantat, Strasbourg, France ULP, Fac Pharm, Lab Immunopharmacol, Strasbourg, France ULP Strasbourg France ac Pharm, Lab Immunopharmacol, Strasbourg, France
Titolo Testata:
HORMONE AND METABOLIC RESEARCH
fascicolo: 8, volume: 31, anno: 1999,
pagine: 448 - 454
SICI:
0018-5043(199908)31:8<448:CAOCSO>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO; CYTOKINE PRODUCTION; MURINE MACROPHAGES; XENOGRAFTS; ALGINATE; TISSUE; MICROENCAPSULATION; BIOCOMPATIBILITY; TRANSPLANTATION; GLUCOCORTICOIDS;
Keywords:
chemotactic factor; bioartificial pancreas; artificial membrane; cell migration; leucocyte;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Kessler, L Serv Endocrinol & Malad Nutr, Pavillon Leriche,1 Pl Hop, F-67091 Strasbourg, France Serv Endocrinol & Malad Nutr Pavillon Leriche,1 Pl HopStrasbourg France F-67091
Citazione:
V. Karsten et al., "Chemotactic activity of culture supernatants of free and encapsulated pancreatic rat islets towards peritoneal macrophages", HORMONE MET, 31(8), 1999, pp. 448-454

Abstract

Longterm efficiency of encapsulated pancreatic islet transplantation is limited by macrophagic reaction at the surface of biocompatible membrane. Theaim of this work was to investigate the influence of soluble factors released by free and encapsulated islets on macrophage chemotaxis. The culture mediums conditioned for 6 days by free and encapsulated rat islets were incubated with peritoneal murine, rat allo and syngenic macrophages to study their migration. Culture supernatants of rat fibroblasts and acinar cells, glucose-stimulated free rat islets and supernatants of free rat islets treated by heat and proteinase K were also tested for their chemotactic activity. Islets encapsulation decreased the chemotactic activity of culture medium conditioned for 6 days by free rat islets on murine (1.66 +/- 0.20 vs. 3.10+/- 0.23; p < 0.001, n = 5) and rat allogenic macrophages (1.63 +/- 0.21 vs. 4.70 +/- 0.36; p < 0.001, n = 9). There was no migration of rat macrophages towards syngenic islets. Fibroblasts exhibited a very strong chemotactic effect as compared to acinar cells. Insulin was not involved in macrophage migration. Proteinase K treatment of culture supernatant of free rat islets totally inhibited the chemotactic activity. After heating at 56 degrees C and 100 degrees C, this activity was reduced to 41 +/- 7% and 32 +/- 5% of the initial activity, respectively. In conclusion, pancreatic islet stimulated macrophage migration by release of immunological specific proteins partly retained by macroencapsulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 03:16:59