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Titolo:
Hyaluronan distribution in the human and canine intervertebral disc and cartilage endplate
Autore:
Inkinen, RI; Lammi, MJ; Agren, U; Tammi, R; Puustjarvi, K; Tammi, MI;
Indirizzi:
Univ Kuopio, Dept Anat, FIN-70211 Kuopio, Finland Univ Kuopio Kuopio Finland FIN-70211 ept Anat, FIN-70211 Kuopio, Finland
Titolo Testata:
HISTOCHEMICAL JOURNAL
fascicolo: 9, volume: 31, anno: 1999,
pagine: 579 - 587
SICI:
0018-2214(1999)31:9<579:HDITHA>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHONDROITIN SULFATE PROTEOGLYCAN; ARTICULAR-CARTILAGE; BINDING REGION; END-PLATE; X COLLAGEN; LOCALIZATION; ACID; CLONING; BRAIN; DOGS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Inkinen, RI Univ Kuopio, Dept Anat, POB 1627, FIN-70211 Kuopio, Finland Univ Kuopio POB 1627 Kuopio Finland FIN-70211 Kuopio, Finland
Citazione:
R.I. Inkinen et al., "Hyaluronan distribution in the human and canine intervertebral disc and cartilage endplate", HISTOCHEM J, 31(9), 1999, pp. 579-587

Abstract

A biotinylated complex of aggrecan G1-domain and link protein was used to characterize the distribution of hyaluronan in paraffin-embedded sections of adult human and canine intervertebral disc and cartilage endplate. Limited chondroitinase ABC and trypsin digestions of the sections before stainingwas utilized to expose hyaluronan potentially masked by aggrecan. Hyaluronan concentration and hyaluronan to uronic acid ratio in different parts of the discs were measured as a background for the histological analysis. Hyaluronan staining was strong in the nucleus pulposus and inner parts of annulus fibrosus of both species, corroborated by biochemical assays of thesame compartments. Particularly in human samples, hyaluronan in the interterritorial matrix of nucleus pulposus and annulus fibrosus was readily accessible to the probe without enzyme treatments. In contrast, the cell-associated hyaluronan signal was enhanced after trypsin or limited chondroitinaseABC-treatment of the sections, suggesting that pericellular hyaluronan wasmore masked by aggrecan than in the distant matrix. A puzzling feature of canine cartilage endplate cells was their intensive cell-associated hyaluronan signal, part of which appeared intracellular. Hyaluronan was abundant between the collagenous lamellae in annulus fibrosus, perhaps important in the plasticity of this tissue.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 10:14:49